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Y1375

YPD Broth

NutriSelect® Basic, powder, for fungi, poly bottle of 250 g, poly bottle of 1 kg

Synonym(s):

Yeast Peptone Dextrose Broth

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171607
Manufacturer/tradename:
NutriSelect® Basic
Ph:
6.3-6.7
Form:
powder
Suitability:
fungi, nonselective for Candida spp., nonselective for Pichia spp., nonselective for Saccharomyces spp., nonselective for Zygosaccharomyces spp.
Application(s):
environmental
food and beverages
microbiology
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sterility

non-sterile

Quality Segment

form

powder

composition

Bacteriological peptone, 20 g/L , Glucose, 20 g/L , Yeast extract, 10 g/L

packaging

poly bottle of 1 kg, poly bottle of 250 g

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

color

beige

pH

6.3-6.7

application(s)

environmental
food and beverages
microbiology

suitability

fungi, nonselective for Candida spp., nonselective for Pichia spp., nonselective for Saccharomyces spp., nonselective for Zygosaccharomyces spp.

General description

YPD Broth is a nutritious general liquid growth medium commonly used for the maintenance and propagation of Pichia pastoris, Saccharomyces cerevisiae, and other yeasts. It is a medium used for molecular cloning, protein expression, or as enrichment media. Peptic digest of animal tissue provides nitrogenous nutrients. Yeast extract provides nitrogenous nutrients as well as Vitamin B Complex. Dextrose provides the carbohydrate and energy source to support the growth of S. cerevisiae.

Application

YPD Broth is recommended for the growth of Saccharomyces cerevisiae for molecular biology purposes. This medium supports the growth of most heterotrophic microorganisms but due to their simple composition, they have been adopted as the basal media for the routine cultivation of yeasts. General methods in yeast genetics specify using Yeast Extract Peptone- Dextrose (YPD) medium for cultivating S. cerevisiae and other yeasts.

Preparation Note

1. Suspend 50 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.
50 g per liter

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Y1375-1KG: + Y1375-250G: + Y1375-VAR: + Y1375-BULK:

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