SCC161 CFBE41o- 6.2 DeltaF508-CFTR Human CF Bronchial Epithelial Cell Line

SCC161
≥1X10⁶ cells/vial   
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      概要

      Replacement Information
      Description
      Catalogue NumberSCC161
      DescriptionCFBE41o- 6.2 DeltaF508-CFTR Human CF Bronchial Epithelial Cell Line
      Alternate Names
      • CFBE41o
      • CF41o
      • CFBE
      Background InformationCystic Fibrosis (CF) is a lethal autosomal recessive disease caused by mutations in the CF transmembrane conductance regulator (CFTR) gene which functions as a cAMP-activated and phosphorylated-regulated Cl channel. The predominant mutation in the CFTR gene is a trinucleotide deletion that results in loss of a phenylalanine at amino acids 508 (ΔF508) in the CFTR protein. This mutation accounts for ~66% of all CF alleles (1).

      CFBE41o- 6.2 ΔF508-CFTR Human CF Bronchial Epithelial Cell line is a subclone derived from the electroporation of the parental CFBE41o- cell line with an Epstein-Barr virus (EBV)-based episomal pCEP4β vector containing the 6.2 kb full length ΔF508 CFTR cDNA and a Hygromycin B resistance gene (1). The 6.2kb ΔF508CFTR cDNA contains the trinucleotide TTT at the ΔF508 locus rather than the naturally occurring CTT and thus makes it possible to differentiate between endogenous ΔF508 CFTR and plasmid derived ΔF508CFTR expression. The 6.2kb ΔF508CFTR cDNA contains both the 5’ and 3’ UTR sequences that are known to affect translational efficiency and mRNA stability. The parental CFBE41o- is a CF human bronchial epithelial cell line, derived from a CF patient homozygous for the ΔF508 CFTR mutation and immortalized with the origin-of-replication defective SV40 plasmid (pSVori-) (1).

      Established CF bronchial epithelial cell lines that are complemented with either wild-type or ΔF508CFTR mRNA will help provide insights into the relationship between transgene-derived CFTR mRNA expression and rescue of cAMP-dependent Cl transport function.

      References:
      1. Illek B, Maurisse R, Wahler L, Kunzelmann K, Fischer H, Gruenert DC. (2008) Cl transport in complemented CF bronchial epithelial cells correlates with CFTR mRNA expression levels. Cell Physiol Biochem 22(1-4): 57-68.
      References
      Product Information
      Applications
      ApplicationCFBE41o- 6.2 ΔF508-CFTR human CF bronchial epithelial cell line may be used to study the relationship between CFTR mRNA expression and Cl transport function.
      Key Applications
      • Cell Culture
      • Cell Based Assays
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Epithelial Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
      • Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, C, and HIV-1 & 2 viruses as assessed by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are negative for mycoplasma contamination.
      • Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications

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