PCRISPR006
Human CRISPR Poison Exon Knockout Library
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この商品について
NACRES:
NE.02
UNSPSC Code:
41105904
packaging
pkg of 5 vials (5x200µL aliquots )
concentration
≥5x108 VP/ml (via p24 Assay)
application(s)
CRISPR
shipped in
dry ice
storage temp.
−70°C
General description
The human, paired guide Poison (pgPoison) library targets 3` splice sites with paired guide RNAs for alternative exon removal (pgRNAs), induce skipping of "poison" cassette exons and corresponding upstream constitutive exons in ultraconserved regions. This compact library (targets 963 exons) allows direct comparison between loss of a constitutive coding exon (knockout) with loss of a poison exon.
Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.
Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.
Application
- Functional Genomics/Target Validation
- Dual-gRNA
- Poison exon
- Internal controls included
Biochem/physiol Actions
In traditional CRISPR KO screens, Cas9 introduces double-strand breaks at locations specified by a gRNA. In this library, a CRISPR-Cas9-based method is used by expressing two sgRNAs to manipulate isoforms independent of gene inactivation. This approach enables rapid suppression of exon recognition in polyclonal settings to identify functional roles for individual exons.
Features and Benefits
- Focus on your research, and we will generate your lentivirus screening library
- Use CRISPR nucleases to knockout protein-coding genes to assess their function.
- Built-in enrichment and depletion controls allow researchers to gauge the success of their pooled screening experiments confidently.
- Dual-Guide Vector System (pools are gRNA-only, Cas9 sold separately) See products: LVCAS9BST or LVCAS9NEO.
- Ease of optimization: Utilizes BFP and Puromycin as selection markers under EF1alpha promoter.
保管分類
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Cartagena Act Listed
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PCRISPR006-1EA: + PCRISPR006-1EA-PW: + PCRISPR006-BULK:
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