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L5135

L-Leucine Dehydrogenase from Bacillus cereus

lyophilized powder, ≥60 units/mg protein

Synonym(s):

L-Leucine:NAD+ oxidoreductase (deaminating)

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About This Item

CAS Number:
9082-71-7
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
1.4.1.9 (BRENDA, IUBMB)
MDL number:
MFCD00147461
Specific activity:
≥60 units/mg protein
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form

lyophilized powder

Quality Segment

200

specific activity

≥60 units/mg protein

mol wt

245 kDa

storage temp.

−20°C

General description

L-Leucine Dehydrogenase is a member of the amino acid dehydrogenase family.

Application

L-Leucine Dehydrogenase from Bacillus cereus has been used to determine the branched-chain amino acids (BCAA) spectrophotometrically in serum samples.

Biochem/physiol Actions

Leucine Dehydrogenase is a nicotinamide adenine dinucleotide hydrogen (NADH)-dependent oxidoreductase. It is involved in catalyzing the reductive amination of aliphatic 2-oxo-acids to their respective L-amino acids.

Other Notes

One unit will convert 1.0 μmole of L‑leucine to α-ketoisocaproate per min at pH 10.5 at 37 °C.
contains lysine

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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