Key Documents

View All Documentation

D9805

Fast Blue B Salt

Name: Fast Blue B Salt
Brand: SIAL

Dye content ~95 %, Powder

Synonym(s):

o-Dianisidine bis(diazotized) zinc double salt, Azoic Diazo No. 48, DBB, Diazo Blue B, Naphthanil Diazo Blue B

Select a Size

Change View

About This Item

Linear Formula:

C₁₄H₁₂N₄O₂Cl₂ · ZnCl₂

CAS Number:

14263-94-6

Molecular Weight:

475.47

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24894113

EC Number:

238-153-2

MDL number:

MFCD00075203

Beilstein/REAXYS Number:

1499067

Colour Index Number:

37235

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Product Name

Fast Blue B Salt, Dye content ~95 %

description

Technical grade

Quality Level

200

form

powder

composition

Dye content, ~95%

technique(s)

microbe id | staining: suitable

color

yellow to dark brown

mp

>300 °C (lit.)

solubility

H₂O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.COc1cc(ccc1[N+]#N)-c2ccc([N+]#N)c(OC)c2

InChI

1S/C14H12N4O2.4ClH.Zn/c1-19-13-7-9(3-5-11(13)17-15)10-4-6-12(18-16)14(8-10)20-2;;;;;/h3-8H,1-2H3;4*1H;/q+2;;;;;+2/p-4

InChI key

GPPKNJIWDULNQH-UHFFFAOYSA-J

Description

Application

Fast Blue B has been used in the acid phosphatase test in Clostridium perfringens.
It has been used as a spraying agent in thin layer chromatography for the identification of separated components.
It has also been used for the determination of the lipase activity.

Biochem/physiol Actions

Fast blue B is used for the determination of acetylcholinesterase activity. Acetylcholinesterase converts 1-naphthyl acetate to 1-naphthol, which reacts with fast blue B to form a purple-coloured diazonium dye. Similarly, fast blue B is also used for α- and β-glucosidase activity.