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Merck

C6164

Cell Freezing Medium-DMSO 1×

sterile-filtered, suitable for cell culture

Synonym(s):

cryopreservation medium

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352207
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sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

impurities

endotoxin, tested

shipped in

dry ice

storage temp.

−20°C

Quality Level

General description

Cell freezing or cryopreservation of cell culture is a routine process. It is essential to maintain the cellular integrity during storage. Majorly used cryoprotectants include glycerol, ethylene glycol and dimethyl sulfoxide(DMSO).

Application

Cell Freezing Medium-DMSO 1× has been used in the cryopreservation of human embryonic kidney HEK293 CNR2 cells, blood mononuclear cells and prostate cancer cells.

Other Notes

Complete ready-to-use medium. Proprietary formulation with Minimum Essential Medium, dimethyl sulfoxide, calf serum and fetal bovine serum.

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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Directional freezing for the cryopreservation of adherent mammalian cells on a substrate
Bahari L, et al.
Testing, 13(2), e0192265-e0192265 (2018)
Variations of components of the plasminogen activation system with the cell cycle in benign prostate tissue and prostate cancer
Plas E, et al.
Cytometry, 46(3), 184-189 (2001)
Antonia Patsialou et al.
Cancer research, 69(24), 9498-9506 (2009-11-26)
Colony-stimulating factor-1 (CSF-1) and its receptor (CSF-1R) have been implicated in the pathogenesis and progression of various types of cancer, including breast cancer. This is based on high levels of circulating CSF-1 in patient sera with aggressive disease and increased
Safety and immunogenicity of oral microencapsulated enterotoxigenic Escherichia coli surface antigen 6 (CS6) with and without LTR192G: a randomized clinical trial
Lapa JA, et al.
Clinical and Vaccine Immunology : CVI (2008)
Sioned M Griffiths et al.
Analytical chemistry, 83(10), 3778-3785 (2011-04-08)
Due to the unique physicochemical properties of nanomaterials (NM) and their unknown reactivity, the possibility of NM altering the optical properties of fluorometric/colorimetric probes that are used to measure their cyto- and genotoxicity may lead to inaccurate readings. This could

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