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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
conjugate
FITC conjugate
Quality Level
form
lyophilized powder
potency
<50 μg/mL agglutination activity
composition
Protein, ~50% Lowry
extent of labeling
~2 mol FITC per mol protein
storage temp.
2-8°C
General description
Lectins are carbohydrate-binding proteins. They are omnipresent and usually found in fungi, plants and animals. The secondary structure of this protein is rich in β-strands and possesses carbohydrate binding sites on the surface.
Application
Lectin from Triticum vulgaris (wheat) has been used:
- to treat transfected cells for immunofluorescence
- to stereotaxically inject into mice for neuronal tracing
- in immunohistochemistry for the analysis of myocytes
For the fluorescent detection of glycoproteins containing β(1→4)-N-acetyl-D-glucosamine
Biochem/physiol Actions
Lectin plays a key role in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. In plants and fungi, lectin defends against pathogens/feeders. Lectin also plays a major role in host recognition and tissue adhesion.
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.
Packaging
Package size based on protein content.
Physical form
Contains phosphate buffer salts
Preparation Note
Conjugates are prepared from affinity purified lectin.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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