Skip to Content
Merck

Key Documents

View All Documentation

09743

3,3′,5,5′-Tetramethylbenzidine

standard for GC

Synonym(s):

BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

Linear Formula:
[-C6H2(CH3)2-4-NH2]2
CAS Number:
54827-17-7
Molecular Weight:
240.34
UNSPSC Code:
41116107
NACRES:
NA.24
PubChem Substance ID:
329748813
EC Number:
259-364-6
Beilstein/REAXYS Number:
2808541
MDL number:
MFCD00007748
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

grade

analytical standard

Quality Level

100

assay

≥99.0% (GC)

shelf life

limited shelf life, expiry date on the label

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

mp

166-171 °C, 168-171 °C (lit.)

application(s)

environmental

format

neat

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

Application

3,3′,5,5′-Tetramethylbenzidine may be used as a substrate for the analysis of diclofenac in water samples and dextromethorphan and its major metabolite dextrorphan in urine samples using enzyme-linked immunosorbent assay (ELISA) and gas chromatography coupled to mass spectrometry (GC-MS).
3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Other Notes

Find a digital Reference Material for this product available on our online platform ChemisTwin® for NMR. You can use this digital equivalent on ChemisTwin® for your sample identity confirmation and compound quantification (with digital external standard). An NMR spectrum of this substance can be viewed and an online comparison against your sample can be performed with a few mouseclicks. Learn more here and start your free trial.
this product will be discontinued, please find the alternative product here :
860336
Standard for determining aromatic amines released from azo-dyes in commodities

Still not finding the right product?

Explore all of our products under 3,3′,5,5′-Tetramethylbenzidine

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCK9666
BCCG9352
BCCD6503
BCCC9345
BCBV5022

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

G. Rohm et al.
GIT Labor-Fachzeit., 1080-1080 (1997)
Development and validation of ELISA and GC-MS procedures for the quantification of dextromethorphan and its main metabolite dextrorphan in urine and oral fluid
Rodrigues CW, et al.
Journal of Analytical Toxicology, 32(3), 220-226 (2008)
Residue analysis of the pharmaceutical diclofenac in different water types using ELISA and GC?MS
Deng A, et al.
Environmental Science & Technology, 37(15), 3422-3429 (2003)
View All Related Papers