P4170
Propidium iodide
≥94.0% (HPLC), fluorescent dye, powder
Synonym(s):
3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide
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About This Item
Empirical Formula (Hill Notation):
C27H34I2N4
CAS Number:
Molecular Weight:
668.39
NACRES:
NA.77
PubChem Substance ID:
UNSPSC Code:
41106305
EC Number:
247-081-0
MDL number:
Beilstein/REAXYS Number:
3843838
Product Name
Propidium iodide, ≥94.0% (HPLC)
Quality Level
assay
≥94.0% (HPLC)
form
powder
mp
220-225 °C (dec.) (lit.)
storage temp.
2-8°C
SMILES string
[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
InChI
1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChI key
XJMOSONTPMZWPB-UHFFFAOYSA-M
General description
Propidium iodide (PI) is a fluorescent dye thatcan be used for staining nucleic acids. It is impermeable in viable orintact cells. It can penetrate easily into dead, dying, or membrane-compromisedcells. PI has the capacity to bind easily to DNA.
Application
Propidiumiodide has been used: to detect cell cycle by flow cytometry and γH2AXand pH3 analysis,apoptosis assay in hepatic stellate cells (HSCs), in stainingbuffer/solution to stain the cells for cell cycle analysis
Features and Benefits
This compound is a featured product for Apoptosis research. Click here to discover more featured Apoptosis products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
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signalword
Warning
hcodes
Hazard Classifications
Muta. 2
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Robin Kumar et al.
Methods in molecular biology (Clifton, N.J.), 2279, 213-223 (2021-03-09)
Annexin V and propidium iodide staining is widely used for determining the cellular death through apoptosis. In the presence of Ca2+ ions, annexin V has a strong binding affinity for phosphatidylserine, a membrane phospholipid that during apoptosis is translocated from
Haiying Liu et al.
Frontiers in cell and developmental biology, 9, 684857-684857 (2021-10-05)
It has been reported that calpain/caspase-mediated apoptosis induced by endoplasmic reticulum stress (ERS) in hepatic stellate cells (HSCs) by previous studies. At present, the activation of HSC is an important cause of liver fibrosis, and the induction of HSC apoptosis
Aswathy Shailaja et al.
Biofilm, 4, 100090-100090 (2022-11-18)
Filamentous fungi are ubiquitous and frequent components of biofilms. A means to visualize them and quantify their viability is essential for understanding their development and disruption. However, quantifying filamentous fungal biofilms poses challenges because, unlike yeasts and bacteria, they are