V900830
DL-Dithiothreitol
Vetec™, reagent grade, 98%
Synonym(s):
threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT
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About This Item
Linear Formula:
HSCH2CH(OH)CH(OH)CH2SH
CAS Number:
Molecular Weight:
154.25
EC Number:
222-468-7
UNSPSC Code:
12352209
PubChem Substance ID:
Beilstein/REAXYS Number:
1719757
MDL number:
grade
reagent grade
product line
Vetec™
assay
98%
mp
41-44 °C (lit.)
storage temp.
2-8°C
SMILES string
O[C@H](CS)[C@H](O)CS
InChI
1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI key
VHJLVAABSRFDPM-QWWZWVQMSA-N
Application
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Legal Information
Vetec is a trademark of Merck KGaA, Darmstadt, Germany
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Qingfang Li et al.
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Objective: Extranodal natural killer /T-cell lymphoma (ENKTL) is an aggressive and unusual subtype of non-Hodgkin lymphoma (NHL) that it is related with the Epstein-Barr virus (EBV). CSF is considered as an ideal source of high-concenrtation disease-related proteins. We aimed at
A A Vorster et al.
Clinical genetics, 87(5), 492-495 (2014-04-29)
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Moshe Ben-David et al.
Journal of molecular biology, 427(6 Pt B), 1359-1374 (2015-02-04)
Despite the abundance of membrane-associated enzymes, the mechanism by which membrane binding stabilizes these enzymes and stimulates their catalysis remains largely unknown. Serum paraoxonase-1 (PON1) is a lipophilic lactonase whose stability and enzymatic activity are dramatically stimulated when associated with