M2279
Minimum Essential Medium Eagle
With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
EMEM, MEM
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Product Name
Minimum Essential Medium Eagle, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
phenol red: yes
sodium pyruvate: no
L-glutamine: no
HEPES: no
NaHCO3: yes
Earle’s salts (5% CO2): yes
shipped in
ambient
storage temp.
2-8°C
General description
Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. It includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for the cultivation of a wide variety of cells grown in monolayers and attached cell lines, such as fibroblasts, in the presence of fetal bovine serum (FBS), calf, or horse sera.
Application
Minimum Essential Medium Eagle has been used to culture the:
- explants of aortic valve leaflets
- human liver cancer cell line (HepG2) cells
- human fetal brain progenitor-derived astrocyte cells (PDA)
- breast cancer cell line (MCF-7)
- human glioblastoma cell line (T98G)
Preparation Note
Supplement with 0.292 g/L L-glutamine.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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