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03-0196-00

SMC® Human Total α-synuclein High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human Total α-Synuclein in human serum, plasma, and cerebrospinal fluid (CSF)

Synonym(s):

Human α-synuclein, α-synuclein, Human total-α-synuclein, a-synuclein, total-a-synuclein

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About This Item

NACRES:
NA.84
UNSPSC Code:
12161503
Species reactivity:
human
Technique(s):
single molecule counting: suitable
Usage:
 kit sufficient for 96 wells
Compatibility:
for use with FemtoQuest™ and SMCxPRO® instruments
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Product Name

SMC® Human Total α-synuclein High Sensitivity Immunoassay Kit,

description

Used for ultrasensitive quantification of Human Total α-Synuclein in human serum, plasma, and cerebrospinal fluid (CSF)

product line

SMC®

usage

 kit sufficient for 96 wells

species reactivity

human

technique(s)

single molecule counting: suitable

input

serum
cerebrospinal fluid (CSF)
plasma (K2 EDTA)

UniProt accession no.

P37840

compatibility

for use with FemtoQuest™ and SMCxPRO® instruments

shipped in

wet ice

storage temp.

2-8°C

General description

α-Synuclein is a small, soluble protein predominantly found in the presynaptic terminals of neurons. It actively participates in synaptic function by regulating neurotransmitter release and maintaining synaptic integrity. Additionally, α-synuclein influences the dynamics of synaptic vesicles and interacts with various proteins involved in synaptic transmission. In neurological disorders, particularly neurodegenerative diseases like Parkinson′s disease, α-synuclein misfolds and aggregates into insoluble fibrils, forming Lewy bodies. These aggregates disrupt cellular function, leading to neuronal toxicity and death. The accumulation of α-synuclein also triggers neuroinflammation by activating microglial cells, which can further contribute to neurodegeneration.

Application

NeuroscienceNeurological DisordersNeurodegenerative DiseasesSynucleinopathies

Features and Benefits

  • Ultra-Sensitive Femtogram Analysis: Achieve exceptionally low detection limits (fg/mL) in serum, plasma and CSF.
  • High-Performance Signal Optimization: Reduced background and expanded dynamic range deliveres highly reproducible results.
  • Intuitive Assay Workflow: Utilize a familiar sandwich immunoassay protocol with next-generation sensitivity.
  • Scalable Automation: Integrate with robotic liquid handling systems to reduce hands on time and improve lab efficiency.
  • Expert Technical Support: Get end-to-end support from field scientists and technical support specialists.

Packaging

96 well assay

Analysis Note

  • Sensitivity: LLOQ 1,950 fg/mL* (SMCxPRO® Instrument)
  • Standard Curve Range: 490-500,000 fg/mL
  • Intra-assay CV: <15%
  • Inter-assay CV: <20%
  • Linearity: Serum: 113% Plasma: 118% CSF: 102%
  • Recovery: Serum: 96% Plasma: 96% CSF: 96%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Other Notes

Although Total α-Synuclein was detectable in non-human primate (rhesus macaque and cynomolgus monkey), feline, rat, mouse, and canine samples, the assay has not been verified for these species.

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
03-0196-0004065265393423