A7906
Bovine Serum Albumin
heat shock fraction, pH 7, ≥98%
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
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About This Item
Biological source:
bovine
Assay:
≥98%
Form:
lyophilized powder
Technique(s):
blood typing: suitable, microbiological culture: suitable
Impurities:
≤5.0% Loss on drying
biological source
bovine
assay
≥98%
form
lyophilized powder
Quality Level
Gene Information
bovine ... ALB(280717)
purified by
heat shock fractionation
packaging
poly bottle of
origin
USA origin
technique(s)
blood typing: suitable, microbiological culture: suitable
impurities
≤5.0% Loss on drying
loss
≤5%
pH
7
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
BT virus, none detected, VSV virus, none detected
storage temp.
2-8°C
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Application
Bovine serum albumin has been used-
- as a component of PCR (polymerase chain reaction) mix
- in the preparation of dissociated cortical neurons
- during immunofluorescent labeling
- as a component of Ca2+-free saline (containing collagenase, hyaluronidase and DNAse) used for bovine tissue digestion
Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Preparation Note
Prepared using heat shock fractionation.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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