01494
Nancy-520
suitable for RT-PCR, ≥90.0% (HPCE)
Greener Alternative
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About This Item
UNSPSC Code:
12171500
EC Number:
200-664-3
NACRES:
NA.32
Quality Segment
assay
≥90.0% (HPCE)
form
liquid
mol wt
78.13 g/mol
sustainability
Sustainability features
color
red to very dark red
bp
189 °C/1,013 hPa
mp
18.4 °C
solubility
DMSO: soluble
density
1.104 g/cm3 at 20 °C
fluorescence
λex 520 nm; λem 554 nm in TE buffer; DNA
storage temp.
2-8°C
General description
This product aligns with one or more of the 12 Principles of Green ChemistryThis product was Designed for Safer Chemistry
Application
Nancy-520 is a fluorescent stain for dsDNA on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is provided as a 5000x stock solution (500 μl), which is sufficient for 50 agarose gels. The limit of detection is 0.5 ng/band of dsDNA.
In addition, Nancy-520 can be used to determine dsDNA concentrations in solution, with a linear range between 0 - 2 μg/ml of DNA. Nancy 520
Nancy-520 has been used successfully in PCR (Real time-PCR) applications.
Nancy 520
Greener alternative to Ethidium bromide.
In addition, Nancy-520 can be used to determine dsDNA concentrations in solution, with a linear range between 0 - 2 μg/ml of DNA. Nancy 520
Nancy-520 has been used successfully in PCR (Real time-PCR) applications.
Nancy 520
Greener alternative to Ethidium bromide.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Storage Class
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
188.6 °F - closed cup - Solvent
Flash Point (°C)
87 °C - closed cup - Solvent
PPE (personal protective equipment)
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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