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Bovine Collagen Type I & III

Name: Bovine Collagen Type I & III
Brand: SIGMA

from bovine hide, powder, suitable for cell culture, PureCol^™

Synonym(s):

Collagen Type I, PureCol Collagen

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.75

EC Number:

232-697-4

Biological source:

bovine

Form:

lyophilized

Technique(s):

cell culture | mammalian: suitable

Assay:

99.9%

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Properties

Product Name

PureCol^™, Collagen powder from Bovine hide, 97% Type I with the remainder of Type III collagen, suitable for cell culture

biological source

bovine

sterility

sterile-filtered

assay

99.9%

form

lyophilized

packaging

pkg of 15 mg

technique(s)

cell culture | mammalian: suitable

binding specificity

Peptide Source: Elastin
, Peptide Source: Fibrinogen

shipped in

wet ice

storage temp.

−20°C

Description

General description

PureCol^™ collagen is approximately 97% Type I with the remainder being comprised of Type III collagen. It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography.

It is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol^™, lyophilized form is not recommended for the formation of a solid gel. PureCol^™ collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.

Application

PureCol^™ has been used:

in collagen gel preparation for the 3D sprouting assay with human umbilical vein endothelial cells (HUVEC) spheroids
to coat Costar^® Transwell^® Inserts plates before cell migration assays with bladder smooth muscle cells (BMSC)
to coat culture plates for collagen degradation assay with breast cancer cell lines

Preparation Note

This product is supplied as a lyophilized powder with 15 mg of bovine collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

Legal Information

Costar is a registered trademark of Corning, Inc.

PureCol is a trademark of Advanced BioMatrix, Inc.

Transwell is a registered trademark of Corning, Inc.

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Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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