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Merck

D7295

Deoxynucleotide Mix, 10 mM

Molecular Biology Reagent

Synonym(s):

Deoxynucleotide Mix, 10 mM, 10mM dNTP mix, dNTP mix, dNTPs

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About This Item

NACRES:
NA.52
UNSPSC Code:
41106305
Assay:
≥99%
Biological source:
synthetic (organic)
Form:
liquid
Solubility:
H2O: soluble at 20 °C
Storage temp.:
−20°C

Key Documents

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biological source

synthetic (organic)

assay

≥99%

form

liquid

concentration

10 mM

color

colorless

solubility

H2O: soluble at 20 °C

application(s)

agriculture

foreign activity

DNase, RNase, none detected

shipped in

dry ice

storage temp.

−20°C

Quality Level

300

Related Categories

General description

Nucleotides are organic molecules that serve as the monomers, or subunits, of nucleic acids (like DNA and RNA). The building blocks of nucleic acids, nucleotides consist of a nitrogenous base (purine or pyrimidine), a five-carbon sugar (ribose or deoxyribose), and at least one phosphate group. A nucleoside along with a phosphate group yields a nucleotide. The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP, and TTP sodium salts in high-quality molecular biology grade water. One µL is sufficient for a standard 50 µL PCR reaction.

Application

dNTP Mix has been used:

  • in the PCR amplification of genomic DNA isolated from insect, fungi, virus, human,
  • in reverse transcription of total RNA to cDNA.
  • as a component of the DNA amplification mixture for polymerase chain reaction (PCR)
  • routine and long PCR
  • manual and automated DNA sequencing
  • cDNA synthesis and labeling reactions

Features and Benefits

  • Purity of each dNTP: Minimum 99%
  • Conveniently formulated; 1 μL is used per 50 μL PCR
  • Equimolar amounts of each dNTP means less pipetting
  • Minimize risk of contamination in PCR
  • UltraPure dNTPs can help maximize consistency and yields in critical PCR reactions

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
WXBF8209V
WXBF8015V
WXBF8461V
WXBF7757V
WXBF7547V

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Abril Sánchez-Botet et al.
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Journal of ethnopharmacology, 244, 112104-112104 (2019-08-09)
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Cancer investigation, 24(5), 484-491 (2006-08-31)
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Zootaxa, 3504(1), 67-88 (2012)
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Virology journal, 18(1), 77-77 (2021-04-17)
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