F5137
Anti-Sheep IgG (whole molecule)−FITC antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Rabbit Anti-Sheep IgG (whole molecule)−Fluorescein isothiocyanate
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About This Item
Conjugate:
FITC conjugate
Clone:
polyclonal
Application:
IF, IHC (p)
Citations:
10
biological source
rabbit
Quality Level
conjugate
FITC conjugate
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
storage condition
protect from light
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, indirect immunofluorescence: 1:100
storage temp.
−20°C
target post-translational modification
unmodified
General description
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Sheep IgG (whole molecule)-FITC antibody is specific for sheep IgG. Rabbit Anti-Sheep IgG is conjugated to Fluorescein Isothiocyanate (FITC) in an alkaline reaction, then further purified to remove unbound FITC.
Immunogen
Purified sheep IgG
Application
Anti-Sheep IgG (whole molecule) FITC antibody produced in rabbit has been used in indirect immunofluorescence assay (IFAT) and immunofluorescence staining.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Related Content
Occurrences of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in sheep from four districts of Tocantins state, Brazilian Legal Amazon Region
Guimaraes A, et al.
Pesquisa Veterinária Brasileira, 35(2), 110-114 (2015)
Yi Wen et al.
American journal of physiology. Renal physiology, 318(1), F107-F116 (2019-11-19)
Nephrotoxic serum nephritis (NTN) models immune-mediated human glomerulonephritis and culminates in kidney inflammation and fibrosis, a process regulated by T lymphocytes. TNF-α is a key proinflammatory cytokine that contributes to diverse forms of renal injury. Therefore, we posited that TNF-α
Yanina P Hecker et al.
Tropical animal health and production, 45(7), 1645-1647 (2013-03-16)
The aim of this study was to describe the occurrence of antibodies to Toxoplasma gondii and Neospora caninum in dairy sheep from the Humid Pampa region, Argentina. Blood samples from 704 dairy sheep belonging to six flocks were collected. Using