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Merck

G0751

β-Glucuronidase from Helix pomatia

Type H-1, partially purified powder, ≥300,000 units/g solid

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
EC Number:
Specific activity:
≥300,000 units/g solid
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type

Type H-1

Quality Segment

form

partially purified powder

specific activity

≥300,000 units/g solid

secondary activity

≥10,000 units/g solid sulfatase

storage temp.

−20°C

General description

β-Glucuronidase Type H-1 from Helix pomatia is partially purified powder containing enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Application

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Biochem/physiol Actions

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Analysis Note

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.


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pictograms

Health hazard

signalword

Danger

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

hcodes

Hazard Classifications

Resp. Sens. 1

wgk

WGK 1



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