G7396
β-Glucuronidase from Escherichia coli
Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay)
Synonym(s):
β-
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
EC Number:
eCl@ss:
32160703
Specific activity:
1,000,000-5,000,000 units/g protein (30 min assay)
Assay:
30—70% (biuret)
Biological source:
Escherichia coli
Quality Segment
biological source
Escherichia coli
type
Type IX-A
assay
30—70% (biuret)
form
lyophilized powder
specific activity
1,000,000-5,000,000 units/g protein (30 min assay)
mol wt
290 kDa
composition
Protein, 30-70% biuret
optimum pH
7—8
shipped in
wet ice
storage temp.
−20°C
General description
Substrate Specificity
a β-D-glucuronoside + H2O = D-glucuronate + an alcohol
a β-D-glucuronoside + H2O = D-glucuronate + an alcohol
Application
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Effective in the hydrolysis of steroid glucuronides.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Analysis Note
Contains buffer salts and stabilizer.
Other Notes
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).
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Signal Word
Danger
Storage Class
11 - Combustible Solids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 1
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