G9880
Glutamic-Pyruvic Transaminase from porcine heart
ammonium sulfate suspension, ≥75 units/mg protein
Synonym(s):
ALT1, L-Alanine: 2-Oxoglutarate Aminotransferase, Alanine Aminotransferase (catalytic concentration), GPT
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About This Item
CAS Number:
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-561-4
MDL number:
Beilstein/REAXYS Number:
3562140
Specific activity:
≥75 units/mg protein
Biological source:
Porcine heart
biological source
Porcine heart
Quality Level
form
ammonium sulfate suspension
specific activity
≥75 units/mg protein
mol wt
42 kDa
foreign activity
L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%, glutamic-oxalacetic transaminase ≤0.05%
shipped in
wet ice
storage temp.
2-8°C
General description
Research area: Cell Signaling
Glutamic-pyruvic transaminase (GPT) is located in most cells in the body but is found in highest concentrations in the liver. Two GPT isoforms, GPT1 and GPT2, are present in mammals.
Glutamic-pyruvic transaminase (GPT) is located in most cells in the body but is found in highest concentrations in the liver. Two GPT isoforms, GPT1 and GPT2, are present in mammals.
Application
Glutamic-Pyruvic Transaminase from porcine heart has been used to determine L-lactate levels in aqueous polyethylene glycol (PEG) solutions.
Biochem/physiol Actions
GPT is responsible for catalyzing the reversible transamination of α-ketoglutarate and alanine to glutamate and pyruvate. It also plays a critical role in the metabolism of amino acids and gluconeogenesis.
Glutamic-pyruvic transaminase (GPT) is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis.
Physical form
Suspension in 1.8 M (NH4)2SO4 solution, pH 6.0
Analysis Note
Protein determined by biuret.
Other Notes
One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.6 at 37 °C in the presence of L-alanine.
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Detection of L-Lactate In Polyethylene Glycol Solutions Confirms Identity of Active Site Ligand in Proline Dehydrogenase Structure
Zhang M and Tanner JJ
Acta Crystallographica Section D, Biological Crystallography, 60(0 5), 985?986-985?986 (2004)
Paradoxical Inhibition of Glycolysis by Pioglitazone Opposes the Mitochondriopathy Caused by AIF Deficiency
Benit P, et al.
EBioMedicine, 17, 75?87-75?87 (2017)
Glutamic-Pyruvic Transaminase 1 Facilitates Alternative Fuels for Hepatocellular Carcinoma Growth?A Small Molecule Inhibitor, Berberine
Guo W, et al.
Cancers, 12(7), 1854-1854 (2020)