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Merck

L2020

Mouse Laminin

from Engelbreth-Holm-Swarm murine sarcoma basement membrane, 0.2 μm filtered, liquid, 1-2 mg/mL, suitable for cell culture

Synonym(s):

Laminin

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About This Item

CAS Number:
114956-81-9
UNSPSC Code:
12352202
NACRES:
NA.75
MDL number:
MFCD00081739
Biological source:
mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)
Form:
aqueous solution
Technique(s):
cell culture | mammalian: suitable
Concentration:
1-2 mg/mL in Tris-buffered saline
Mol wt:
A subunit 400 kDa, B1 subunit 210 kDa, B2 subunit 200 kDa

Key Documents

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Product Name

Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane, 1-2 mg/mL in Tris-buffered saline, 0.2 μm filtered, BioReagent, suitable for cell culture

biological source

mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)

product line

BioReagent

form

aqueous solution

mol wt

A subunit 400 kDa, B1 subunit 210 kDa, B2 subunit 200 kDa

packaging

pkg of 1 mg

concentration

1-2 mg/mL in Tris-buffered saline

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑2 μg/cm2

impurities

Microbial Contamination, passes test

NCBI accession no.

NM_008480.2

UniProt accession no.

P02468, P02469, P19137, Q61092

binding specificity

Peptide Source: Collagen

shipped in

dry ice

storage temp.

−20°C

Quality Level

300

Gene Information

mouse ... Lama1(16772), Lamb1(16777), Lamc1(226519), Lamc2(16782)

Related Categories

General description

Laminins are non-collagenous adhesive glycoproteins with a substantial molecular weight. They are heterotrimeric proteins with three disulfide-linked polypeptides known as the α, β, and γ chains. The multiple polypeptide domains of laminins can bind to multiple cell surface receptors, including several integrin dimers, dystroglycan and syndecans, and other ECM components. This extracellular matrix multidomain trimeric glycoprotein is the main component of basal lamina that supports adhesion, proliferation and differentiation.

Application

Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used in:
  • insulin-producing cell (IPC) differentiation.
  • Infection inhibition assays.
  • Proliferation Assay.
Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.

Biochem/physiol Actions

Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment.

Preparation Note

This product is supplied at a concentration of 1 − 2 mg/mL in 50 mM Tris-HCl, with 150 mM NaCl. It is filtered using a 0.2μm filter. Thaw this solution slowly before use at 2-8°C to avoid gel formation. For use as a coating, dilute in a balanced salt solution, coat culture surface with a minimal volume and incubate at 37°C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8°C.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product was isolated from mouse Engelbreth-Holm-Swarm tumor.

Disclaimer

It is recommended to store this product in working aliquots at -20°C.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000519327
0000519327
0000488838
0000488838
0000464593

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Mitogenic activity of laminin on human melanoma and melanocytes: different signal requirements and role of beta 1 integrins.
Mortarini R, et al.
Cancer Research, 55(20), 4702-4710 (1995)
Axonal pathfinding: Extracellular matrix role
Letourneau, P
Encyclopedia of Neuroscience, 1, 1139-1145 (2009)
Serotype-specific entry of dengue virus into liver cells: identification of the 37-kilodalton/67-kilodalton high-affinity laminin receptor as a dengue virus serotype 1 receptor.
Thepparit C and Smith DR.
Journal of Virology, 78(22), 12647-12656 (2004)
Agnese Loda et al.
Nature communications, 8(1), 690-690 (2017-09-28)
Xist is indispensable for X chromosome inactivation. However, how Xist RNA directs chromosome-wide silencing and why some regions are more efficiently silenced than others remains unknown. Here, we explore the function of Xist by inducing ectopic Xist expression from multiple
Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK.
Lin HY, et al.
Journal of Biomedical Science, 17:56 (2010)
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Laminin Coating Protocol for Cell Culture

Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.

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