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L3287

Escort^™ IV Transfection Reagent

Name: Escort<SUP>™</SUP> IV Transfection Reagent
Brand: SIGMA

Lipid reagent for transient and stable transfection of mammalian and insect cells.

Synonym(s):

Gene delivery

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About This Item

NACRES:

NA.25

UNSPSC Code:

41106502

Form:

liquid (aqueous solution)

Grade:

Molecular Biology

Technique(s):

transfection: suitable

Concentration:

1 mg/mL

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Properties

grade

Molecular Biology

Quality Level

100

form

liquid (aqueous solution)

usage

mL sufficient for 160-500 transfections

concentration

1 mg/mL

technique(s)

transfection: suitable

storage temp.

2-8°C

Description

General description

Escort^™ IV is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into a wide variety of eukaryotic cell types.

Application

Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 4-16 μL Escort^™ IV and 2 μg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. For a list of cells that have been successfully transfected using Escort^™ IV, see the Transfection Reagent Selection Guide.

Biochem/physiol Actions

A stable complex is formed when Escort^™ IV is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.

Features and Benefits

Suitable for stable and transient transfection
Optimized for a wide variety of cell lines
Low toxicity
Compatible with both serum and serum-free transfection protocols
Ideal for Sf9, Sf21 and S2 insect cells

Other Notes

Escort^™ IV formulation:
1 mg/mL total lipid in water

Note the identity of the lipids used in Escort^™ IV is confidential.

Legal Information

Escort is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

Do not freeze.

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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Protocols

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

Escort™ IV Transfection Reagent Protocol

Product manual provides detailed protocol for easy DNA transfection.

Escort™ III Transfection Reagent Protocol

The product bulletin providin detailed use protocol for easy DNA transfection.

View All Protocols

Articles

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

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