NA0200

GenElute^™ HP Plasmid Midiprep Kit

Name: GenElute<SUP>™</SUP> HP Plasmid Midiprep Kit
Brand: SIGMA

sufficient for 25 purifications

Synonym(s):

GenElute^™ HP Plasmid Midiprep Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, Gen Elute

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About This Item

NACRES:

NA.52

UNSPSC Code:

41105501

Key Documents

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usage

sufficient for 25 purifications

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

DNA purification: suitable

Quality Level

200

test parameters

sample volume: 1-5 mL bacterial culture

greener alternative category

, Aligned

storage temp.

15-25°C

Related Categories

Greener Alternative Products

Nucleic Acid Purification

Plasmid DNA | Plasmid Purification Kits

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

The GenElute HP Plasmid Midiprep Purification kits offer an ultrafast and efficient solution for large-scale plasmid preparation from E. coli cultures. These kits combine silica-based membrane technology and the convenience of spin or vacuum format. The kits also include a special filter cartridge, which replaces the centrifugation step following alkaline lysis. Following lysis the DNA is bound to the silica membrane and contaminants are removed with a simple wash step. The DNA is then eluted in water or TE buffer by centrifugation.

The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.

Click here to view the kit protocol

Application

GenElute^™ HP Plasmid Midiprep Kit has been used to isolate and purify plasmids.

Features and Benefits

From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
Up to 350 μg of high-copy plasmid DNA
Flexibility of a vacuum or spin format
No phenol/chloroform extraction or alcohol precipitation required
Contains fewer plastic components, reducing the amount of waste

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.

Description

SDS

R1149Resuspension Solution, Add RNase A before use. After addition of RNAse, store at 2-8ºC. )SDS

W0263Wash Solution 1SDS

W4639Wash Solution 2SDS

R6148RNase A solutionSDS

C2112Column Preparation SolutionSDS

L1912Lysis SolutionSDS

pictograms

GHS02,GHS08,GHS05,GHS07

signalword

Danger

pcodes

P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338

target_organs

Central nervous system

Storage Class

3 - Flammable liquids

wgk

WGK 3

hcodes

H226,H290,H302 + H332,H314,H334,H336

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

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Elucidating the Molecular Determinants of Aβ Aggregation with Deep Mutational Scanning.

Vanessa E Gray et al.

G3 (Bethesda, Md.), 9(11), 3683-3689 (2019-09-29)

Despite the importance of Aβ aggregation in Alzheimer's disease etiology, our understanding of the sequence determinants of aggregation is sparse and largely derived from in vitro studies. For example, in vitro proline and alanine scanning mutagenesis of Aβ40 proposed core

Genome-wide CRISPR screens reveal a Wnt-FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors.

Steinhart Z

Nature Medicine, 23(1), 60-68 (2017)

The distal upstream promoter in Ly49 genes, Pro1, is active in mature NK cells and T cells, does not require TATA boxes, and displays enhancer activity.

Gays F

Journal of Immunology, 194(12), 6068-6081 (2015)

Rapid expression and purification of the hepatitis delta virus antigen using the methylotropic yeast Pichia pastoris.

Stephanie P Cartwright et al.

BMC research notes, 10(1), 340-340 (2017-07-29)

Patients with dual hepatitis B (HBV) and hepatitis D (HDV) virus infection are at an increased risk of progression to liver cirrhosis and hepatocellular carcinoma than patients with a single viral infection. Treatment of viral hepatitis due to dual HBV/HDV

Multiplexed measurement of variant abundance and activity reveals VKOR topology, active site and human variant impact.

Melissa A Chiasson et al.

eLife, 9 (2020-09-02)

Vitamin K epoxide reductase (VKOR) drives the vitamin K cycle, activating vitamin K-dependent blood clotting factors. VKOR is also the target of the widely used anticoagulant drug, warfarin. Despite VKOR's pivotal role in coagulation, its structure and active site remain

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GenElute^™ HP Plasmid Midiprep Kit