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Merck

P4170

Propidium iodide

≥94.0% (HPLC), fluorescent dye, powder

Synonym(s):

3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide

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About This Item

Empirical Formula (Hill Notation):
C27H34I2N4
CAS Number:
25535-16-4
Molecular Weight:
668.39
NACRES:
NA.77
PubChem Substance ID:
57654524
UNSPSC Code:
41106305
EC Number:
247-081-0
MDL number:
MFCD00011921
Beilstein/REAXYS Number:
3843838

Key Documents

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Product Name

Propidium iodide, ≥94.0% (HPLC)

InChI key

XJMOSONTPMZWPB-UHFFFAOYSA-M

InChI

1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1

SMILES string

[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

assay

≥94.0% (HPLC)

form

powder

mp

220-225 °C (dec.) (lit.)

storage temp.

2-8°C

Quality Level

200

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General description

Propidium iodide (PI) is a fluorescent dye thatcan be used for staining nucleic acids. It is impermeable in viable orintact cells. It can penetrate easily into dead, dying, or membrane-compromisedcells. PI has the capacity to bind easily to DNA.

Application

Propidiumiodide has been used: to detect cell cycle by flow cytometry and γH2AXand pH3 analysis,apoptosis assay in hepatic stellate cells (HSCs), in stainingbuffer/solution to stain the cells for cell cycle analysis

Features and Benefits

This compound is a featured product for Apoptosis research. Click here to discover more featured Apoptosis products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

pictograms

Health hazard
GHS08

signalword

Warning

hcodes

H341

Hazard Classifications

Muta. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
WXBF7582V
WXBF6662V
WXBF6053V
WXBF5497V
WXBF4936V

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Haiying Liu et al.
Frontiers in cell and developmental biology, 9, 684857-684857 (2021-10-05)
It has been reported that calpain/caspase-mediated apoptosis induced by endoplasmic reticulum stress (ERS) in hepatic stellate cells (HSCs) by previous studies. At present, the activation of HSC is an important cause of liver fibrosis, and the induction of HSC apoptosis
Aswathy Shailaja et al.
Biofilm, 4, 100090-100090 (2022-11-18)
Filamentous fungi are ubiquitous and frequent components of biofilms. A means to visualize them and quantify their viability is essential for understanding their development and disruption. However, quantifying filamentous fungal biofilms poses challenges because, unlike yeasts and bacteria, they are
Robin Kumar et al.
Methods in molecular biology (Clifton, N.J.), 2279, 213-223 (2021-03-09)
Annexin V and propidium iodide staining is widely used for determining the cellular death through apoptosis. In the presence of Ca2+ ions, annexin V has a strong binding affinity for phosphatidylserine, a membrane phospholipid that during apoptosis is translocated from
Julia Burrill et al.
Forensic science international. Genetics, 46, 102269-102269 (2020-03-11)
Forensic DNA typing from touched or handled items in routine casework is increasing as the sensitivity of detection techniques improves. Our understanding of the cellular/acellular content of touch deposits and the origins of the DNA therein is still limited. This
Wei Liang et al.
Molecular immunology, 112, 312-321 (2019-06-24)
Precise glycosylation plays a crucial and distinctive role in thymic T cell development. The core fucosylation is dramatically up-regulated at the transition from CD4-CD8- (DN) to CD4+CD8+ (DP) in the thymic development. Ablation of core fucosylation in T cells did
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