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S1797

SOC Medium

Name: SOC Medium
Brand: SIGMA

For use in transformation

Synonym(s):

Super Optimal broth

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About This Item

NACRES:

NA.85

UNSPSC Code:

41106200

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Properties

grade

Molecular Biology

Quality Level

500

sterility

0.2 μm filtered

form

liquid

composition

Dextrose, 3.603 g/L , KCl, 0.186 g/L , MgSO₄, 4.8 g/L , Tryptone, 20 g/L , Yeast extract, 5 g/L

technique(s)

microbiological culture: suitable

application(s)

agriculture

storage temp.

2-8°C

suitability

nonselective for Escherichia coli, nonselective for coliforms

Description

General description

Super optimal medium with catabolic repressor (SOC) medium is a glucose-rich microbial growth medium used primarily in the recovery step of Escherichia coli competent cell transformations. The culture of E.coli cells in this nutrient-rich microbial broth increases the transformation efficiency of recombinant plasmids containing toxic protein genes by inhibiting the unintended activation of the lac promoter carried on the cloning vector. SOC medium contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.

Application

SOC Medium has been used for the transformation of DH5α competent E-coli cells. It has also been used to culture white colonies post-cloning for the identification of the recombinant protein of interest.

Features and Benefits

SOC liquid provides:

Filter-sterilized, ready-to-use format
Convenient package sizes
Standard formulation

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Protocols

Competent Cell Protocols

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

Articles

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).