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Merck

W3500

Water

sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

Cell culture grade water, Ultra-filtered Water

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About This Item

Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
EC Number:
231-791-2
Beilstein/REAXYS Number:
2050024
MDL number:
Technique(s):
cell culture | mammalian: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg
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vapor density

<1 (vs air)

Quality Level

vapor pressure

3 mmHg

sterility

sterile-filtered

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/mL endotoxin

refractive index

n20/D 1.34 (lit.)

pH

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

General description

This product is a very low endotoxin (less than 1 EU/mL), 0.2 micron filtered.

Application

For use in the preparation of cell culture media, and cell suspension and washing solutions.

Other Notes

Easily compare specifications for Water products with the Water specification table


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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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Protocols

Preparation from Packaged Powder, Preparation from Basal Salt Solutions, Banana Powder Preparation and Use, Coconut Water Preparation and Use

Articles

균체 내독소란 무엇입니까? 체외 세포 배양에서의 균체 내독소 오염에 관한 자주 묻는 질문입니다. LAL 분석을 사용한 내독소 검사 방법, 실험실 내독소 오염의 공통 출처와 세포 라인을 배양할 때 내독소 오염을 피하는 방법에 대한 자세한 내용입니다.

FAQs on bacterial endotoxin contamination, details on endotoxin testing using the LAL assay, and tips to avoid contamination in cell cultures.

High-quality ultrapure water that is endotoxin-free is recommended for mammalian cell culture as it support cell viability and prevents contamination.


Philippe Robert et al.
Investigative radiology, 50(8), 473-480 (2015-06-25)
To prospectively compare in healthy rats the effect of multiple injections of macrocyclic (gadoterate meglumine) and linear (gadodiamide) gadolinium-based contrast agents (GBCAs) on T1-weighted signal intensity in the deep cerebellar nuclei (DCN), including the dentate nucleus. Healthy rats (n =
Mary A Rodgers et al.
The Journal of experimental medicine, 211(7), 1333-1347 (2014-06-25)
Linear ubiquitination is a newly discovered posttranslational modification that is currently restricted to a small number of known protein substrates. The linear ubiquitination assembly complex (LUBAC), consisting of HOIL-1L, HOIP, and Sharpin, has been reported to activate NF-κB-mediated transcription in
Susu Duan et al.
Nature communications, 5, 5029-5029 (2014-10-10)
Oseltamivir-resistant H1N1 influenza viruses carrying the H275Y neuraminidase mutation predominated worldwide during the 2007-2009 seasons. Although several neuraminidase substitutions were found to be necessary to counteract the adverse effects of H275Y, the order and impact of evolutionary events involved remain