biological source
rabbit
Quality Segment
conjugate
ALEXA FLUOR™ 488
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, mouse
species reactivity (predicted by homology)
human (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TUBB3(10381)
mouse ... Tubb3(22152)
rat ... Tubb3(246118)
General description
50 kDa calculated
Tubulin is one of several members of a small family of globular proteins which polymerize to form cytoskeletal structures called microtubules. The most common members of the tubulin family are α-tubulin and β-tubulin. Tubulin dimers, typically composed of α- and β-tubulin subunits, assemble onto microtubules forming large polymers. β-III tubulin is a unique microtubule subunit that is found expressed almost exclusively in neurons. Due to the unique expression pattern of β-III tubulin it is often utilized as a marker to identify neuronal cells from other brain cells such as glia which do not express it. β-III tubulin also serves as a cytoarchitectural marker since it is primarily found within neurite extensions.
Immunogen
Synthetic linear peptide corresponding to human Beta III Tubulin.
Application
Immunohistochemistry: Representive lot has been shown to work in adult mouse brain.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism
Neurofilament & Neuron Metabolism
Biochem/physiol Actions
This antibody recognizes Beta III Tubulin.
Physical form
Affinity purified
Purified rabbit polyclonal conjugated to Alexa Fluor™ 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat E18 cortex cells
Rat E18 cortex cells
Evaluated by Immunocytochemistry in rat E18 cortex cells.
Immunocytochemistry Analysis: A 1:400 dilution of this antibody detected Beta III Tubulin in rat E18 cortex cells.
Immunocytochemistry Analysis: A 1:400 dilution of this antibody detected Beta III Tubulin in rat E18 cortex cells.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK 2
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