Anti-phospho-erbB2 (Thr686) Antibody, clone 7F8

180 kDa

KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 686 in human erbB2

Anti-phospho-erbB2 (Thr686) Antibody, clone 7F8 is an antibody against phospho-erbB2 (Thr686) for use in ELISA & WB.

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

Kinases & Phosphatases

Recognizes phosphorylated erbB2 on Threonine 686

Format: Purified

In 1 mL of PBS containing 0.09% sodium azide, PEG, sucrose and 50% glycerol.

Subsequent thiophilic adsorption and size exclusion chromatography

2 years at -20°C from date of shipment. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Routinely evaluated by immunoblot.

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