05-1352
Anti-Histone H2B Antibody, clone 5HH2-2A8
ascites fluid, clone 5HH2-2A8, from mouse
동의어(들):
H2B, Histone H2B, histone 2, H2be, histone cluster 2, H2be, H2B histone family, member Q
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
5HH2-2A8, monoclonal
Application:
ICC, WB
Citations:
7
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
5HH2-2A8, monoclonal
species reactivity
mouse, human, rat
technique(s)
immunocytochemistry: suitable, western blot: suitable
isotype
IgG1κ
GenBank accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... H2BC21(8349)
mouse ... H2Bc21(319190)
General description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2B is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
~15 kDa
Immunogen
Epitope: C-terminus
Ovalbumin-conjugated synthetic linear peptide corresponding to the C-terminus of Histone H2B.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Anti-Histone H2B Antibody, clone 5HH2-2A8 is a Mouse Monoclonal Antibody for detection of Histone H2B also known as Histone H2B, histone 2 H2be & has been validated in WB, ICC.
Immunocytochemistry:
A 1:500 dilution of a representative lot of this antibody detected Histone H2B in A431, HeLa and NIH/3T3 cells.
A 1:500 dilution of a representative lot of this antibody detected Histone H2B in A431, HeLa and NIH/3T3 cells.
Biochem/physiol Actions
Antibody recognizes Histone H2B near the C-terminus.
Physical form
Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HepG2 cell lysate, L6 cell lysate, Mouse Brain Lysate
HepG2 cell lysate, L6 cell lysate, Mouse Brain Lysate
Evaluated by western blot in HepG2 cell lysate.
Western Blot Analysis:
A 1:1,000-1:2,000 dilution of this antibody detected Histone H2B in 10 µg of HepG2 cell lysate.
Western Blot Analysis:
A 1:1,000-1:2,000 dilution of this antibody detected Histone H2B in 10 µg of HepG2 cell lysate.
Other Notes
Replaces: MABE15
Legal Information
GenBank is a registered trademark of United States Department of Health and Human Services
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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저장 등급
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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Lecona, E; Narendra, V; Reinberg, D
Molecular and cellular biology null
Usha P Andley et al.
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Understanding the mechanisms of cataract formation is important for age-related and hereditary cataracts caused by mutations in lens protein genes. Lens proteins of the crystallin gene families α-, β-, and γ-crystallin are the most abundant proteins in the lens. Single
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Valery F Thompson et al.
Biochemistry, 57(51), 7021-7032 (2018-11-30)
Purified recombinant FUsed in Sarcoma (FUS) assembles into an oligomeric state in an RNA-dependent manner to form large condensates. FUS condensates bind and concentrate the C-terminal domain of RNA polymerase II (RNA Pol II). We asked whether a granule in
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European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology, 39, 29-45 (2020-09-08)
The sigma-1 receptor (S1R) is a membrane-associated protein expressed in neurons and glia at mitochondria-associated endoplasmic reticulum (ER) membranes (MAMs). S1R interacts with different partners to regulate cellular responses, including ER stress, mitochondrial physiology and Ca2+ fluxes. S1R shapes cellular
문서
Loading controls in western blotting application.
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| 05-1352 | 04053252628726 |
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