biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
20G3, monoclonal
species reactivity
human, mouse
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (non-phospho-Tyr1173)
Gene Information
human ... EGFR(1956)
mouse ... Egfr(13649)
General description
175kDa
Immunogen
peptide containing the unphosphorylated Tyr1173 motif (NAE[Y]LR) of the human EGF receptor
Application
Detect EGFR (non-phospho-Tyr1173) using this Anti-EGFR (non-phospho-Tyr1173) Antibody, clone 20G3 validated for use in IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Biochem/physiol Actions
The motif NAEYLR of the EGF-R (unphosphorylated at Tyr1173), this antibody does not interact with the phosphorylated (pTyr1173) form of the EGF receptor. Tyr1173 corresponds to Tyr1197 in the human (isoform a) and mouse precursor proteins, and to Tyr1196 in the mature rat and pig precursor proteins.
Physical form
100µg of lyophilized thiophilic and size exclusion chromatography purified mouse IgG1 from 1mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 100 µL H2O (15 min, RT) for a final concentration of 1 mg/mL.
Format: Purified
Thiophilic adsoption and size exclusion chromatography
Preparation Note
2 years at -20°C
Analysis Note
routinely evaluated by immunoblot on RIPA lysates from unstimulated HFF cells
Other Notes
Replaces: 04-337; 04-341
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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10 - Combustible liquids
wgk
WGK 1
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