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Merck

05-499

Anti-Histone H3.1t (H3-4) Antibody

UPSTATE®, mouse monoclonal, 6.6.2

동의어(들):

H3 histone, family 3A

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
6.6.2, monoclonal
Species reactivity:
vertebrates, human, plant
Application:
WB
Citations:
168
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제품 이름

Anti-Histone H3 Antibody, clone 6.6.2, clone 6.6.2, Upstate®, from mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6.6.2, monoclonal

species reactivity

vertebrates, human, plant

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... H3F3B(3021)

General description

17 kDa
This monoclonal antibody was generated using an unmodified peptide corresponding to the first 20 amino acids of histone H3 and is specific for an unmodified epitope in that region.

Immunogen

Epitope: a.a. 1-20
KLH conjugated peptide corresponding to amino acids 1-20 [ARTKQTARKSTGGKAPRKQLC] of human histone H3. Clone 6.6.2.

Application

Anti-Histone H3 Antibody, clone 6.6.2 is a Mouse Monoclonal Antibody for detection of Histone H3 also known as H3 histone family 3A & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Biochem/physiol Actions

This peptide sequence is identical in a wide range of animal and plant species.
This antibody recognizes and is specific for histone H3, Mr 17kDa.

Physical form

Format: Purified
Protein G Chromatography
Purified mouse monoclonal IgG1κ in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, with 0.05% sodium azide and 30% glycerol.

Preparation Note

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.

Analysis Note

Control
HeLa cell extract
Routinely evaluated by western blot on acid extracted lysates from HeLa cells.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected Histone H3 in acid extracted lysates from HeLa cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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저장 등급

10 - Combustible liquids

wgk

WGK 1


시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

A HIGHLY SELECTIVE OLIGOPEPTIDE BINDING PROTEIN FROM THE ARCHAEON SULFOLOBUS SOLFATARICUS.
Gogliettino M, Balestrieri M, Pocsfalvi G, Fiume I, Natale L, Rossi M, Palmieri G
Journal of Bacteriology null
Induction of human fetal hemoglobin via the NRF2 antioxidant response signaling pathway.
Macari, ER; Lowrey, CH
Blood null
Phase I pharmacokinetic and pharmacodynamic evaluation of combined valproic acid/doxorubicin treatment in dogs with spontaneous cancer.
Wittenburg LA, Gustafson DL, Thamm DH
Clinical cancer research : an official journal of the American Association for Cancer Research null
Glycosylation and sialylation of macrophage-derived human apolipoprotein E analyzed by SDS-PAGE and mass spectrometry: evidence for a novel site of glycosylation on Ser290.
Lee Y, Kockx M, Raftery MJ, Jessup W, Griffith R, Kritharides L
Molecular and Cellular Proteomics null
Andreas Ratzka et al.
Journal of neurochemistry, 122(1), 94-105 (2012-04-28)
Fibroblast growth factor 2 (FGF-2) is a neurotrophic factor participating in regulation of proliferation, differentiation, apoptosis and neuroprotection in the central nervous system. With regard to dopaminergic (DA) neurons of substantia nigra pars compacta (SNpc), which degenerate in Parkinson's disease

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05-49904053252403675

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