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Merck

07-030

Anti-dimethyl-Histone H3 (Lys4) Antibody

Upstate®, from rabbit

동의어(들):

H3K4me2, Histone H3 (di methyl K4), H3 histone family, member T, histone 3, H3, histone cluster 3, H3

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
Tetrahymena sp., human
Application:
ChIP, DB, ICC, IF, WB, inhibition assay
Citations:
988

주요 문서

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기술 서비스
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도움 문의

biological source

rabbit

antibody form

unpurified

antibody product type

primary antibodies

clone

polyclonal

species reactivity

Tetrahymena sp., human

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable (ChIP-seq), dot blot: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, inhibition assay: suitable (peptide), western blot: suitable

NCBI accession no.

NP_003484.1

UniProt accession no.

Q16695

shipped in

dry ice

target post-translational modification

dimethylation (Lys4)

Quality Level

100

Gene Information

human ... H3C1(8350)

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Approx. 17 kDa

Immunogen

Epitope: dimethylated Histone H3 (Lys4)
KLH conjugated synthetic peptide (ARTMe2KQTAR-GC) corresponding to amino acids 1-8 of human Histone H3.

Application

Chromatin Immunoprecipitation:
5 μL of a previous lot immunoprecipitated methylated Histone H3 from 1 x 106 3T3/A31 cells.

Immunocytochemistry:
1:500 dilution of a previous lot showed positive immunostaining for methylated Histone H3 in L6 cells fixed with 4% paraformaldehyde and permeabilized using 0.1% Triton X-100.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-dimethyl-Histone H3 (Lys4) Antibody (rabbit polyclonal antibody) is published and validated in ChIP, DB, ICC, IF, WB, PIA, ChIP-seq. to detect dimethyl-Histone H3 (Lys4) also known as H3K4me2, Histone H3 (di methyl K4).

Biochem/physiol Actions

Broad species cross-reactivity is expected.
Recognizes dimethylated Histone H3 (Lys4), MW ~17 kDa. Specificity demonstrated by ELISA using methylated peptides.

Physical form

Format: Unpurified
Rabbit polyclonal antiserum containing 0.05% sodium azide.
Unpurified

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HeLa acid extract
Evaluated by western blot on HeLa acid extract.

Western Blot Analysis: 1:2000-1:10,000 dilution of this antibody detected methylated Histone H3 in acid extracted proteins from HeLa cells.
1:2000-1:5000 dilution of a previous lot detected methylated Histone H3 in acid extracted proteins from HeLa and tetrahymena cells.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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저장 등급

10 - Combustible liquids

wgk

WGK 1

시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

The requirements for COMPASS and Paf1 in transcriptional silencing and methylation of histone H3 in Saccharomyces cerevisiae.
Mueller, JE; Canze, M; Bryk, M
Genetics null
Pharmacologic inhibition of epigenetic modifications, coupled with gene expression profiling, reveals novel targets of aberrant DNA methylation and histone deacetylation in lung cancer.
Zhong, S; Fields, CR; Su, N; Pan, YX; Robertson, KD
Oncogene null
Dorota Skowronska-Krawczyk et al.
Development (Cambridge, England), 131(18), 4447-4454 (2004-09-03)
Basic helix-loop-helix (bHLH) transcription factors such as atonal homolog 5 (ATH5) and neurogenin 2 (NGN2) determine crucial events in retinogenesis. Using chromatin immunoprecipitation, we demonstrate that their interactions with target promoters undergo dynamic changes as development proceeds in the chick
Epigenetic changes to the MDR1 locus in response to chemotherapeutic drugs.
Baker, EK; Johnstone, RW; Zalcberg, JR; El-Osta, A
Oncogene null
Distribution of histone H3.3 in hematopoietic cell lineages.
Jin, C; Felsenfeld, G
Proceedings of the National Academy of Sciences of the USA null
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관련 콘텐츠

Shaping Epigenetics Discovery - Epigenetics Product Selection Brochure

"Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms instead of by alterations in DNA sequence. These changes can be cell- or tissue-specific, and can be passed on to multiple generations. Epigenetic regulation enriches DNAbased information, allowing a cell to vary its response across diverse biological and environmental contexts. Although epigenetic mechanisms are primarily centered in the nucleus, these mechanisms can be induced by environmental signals such as hormones, nutrients, stress, and cellular damage, pointing to the involvement of cytoplasmic and extracellular factors in epigenetic regulation."

Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

Signaling Product Guide: Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

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SKUGTIN
07-03004053252269202

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