07-449

Anti-trimethyl-Histone H3 (Lys27) Antibody

Name: Anti-trimethyl-Histone H3 (Lys27) Antibody
Brand: MM

Upstate^®, from rabbit

동의어(들):

H3 histone family, member T, histone 3, H3, histone cluster 3, H3, H3k27me3

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Clone:

polyclonal

Species reactivity:

human, mouse

Application:

ICC, IHC, IP, WB, multiplexing

Citations:

2261

주요 문서

모든 문서 보기

기술 서비스

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도움 문의

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도움 문의

biological source

rabbit

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse

manufacturer/tradename

Upstate^®

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, multiplexing: suitable, western blot: suitable

isotype

IgG

NCBI accession no.

NM_003493

UniProt accession no.

Q16695

shipped in

dry ice

target post-translational modification

trimethylation (Lys27)

Quality Level

100

Gene Information

human ... HIST1H3F(8968)

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General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

17 kDa

Immunogen

KLH-conjugated, synthetic 2X-branched peptide containing the sequence …AR(me3K)SAP… in which me3K corresponds to trimethyl-lysine at residue 27 of human histone H3.

Application

Anti-trimethyl-Histone H3 (Lys27), also known as Anti-H3K27me3, is a highly published Rabbit Polyclonal Antibody. This protein A purified antibody is dot blot tested for trimethylated lysine 27 specificity and validated in WB, ICC, IP.

Immunocytochemistry:

Immunoprecipitation:

Beadlyte Histone-Peptide Specificity Assay:

Biochem/physiol Actions

Trimethylated histone H3 (Lys27). Broad species cross-reactivity expected.

Broad species cross-reactivity expected.

Physical form

Format: Purified

Purified rabbit IgG in buffer containing 0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide before the addition of 30% glycerol.

Analysis Note

Routinely evaluated by western blot in acid extracted proteins from HeLa cells.

Western Blot Analysis:
0.4 µg/mL of anti-trimethyl histone H3 Lys27 was used on recombinant Histone H3 and HeLa cell acid extracts.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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저장 등급

10 - Combustible liquids

wgk

WGK 1

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국제 무역 품목 번호

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07-449	04053252671517

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Anti-trimethyl-Histone H3 (Lys27) Antibody

Upstate®, from rabbit

크기 선택

제품정보 (DICE 배송 시 비용 별도)

주요 문서

속성

biological source

antibody form

antibody product type

clone

species reactivity

manufacturer/tradename

technique(s)

isotype

NCBI accession no.

UniProt accession no.

shipped in

target post-translational modification

Quality Level

Gene Information

설명

General description

Immunogen

Application

Biochem/physiol Actions

Physical form

Analysis Note

Other Notes

Legal Information

안전성 정보

저장 등급

wgk

문서

시험 성적서(COA)

이 제품을 이미 가지고 계십니까?

관련 참고 논문

관련 참고 프로토콜

문서

프로토콜

관련 콘텐츠

국제 무역 품목 번호

기술 서비스

Upstate^®, from rabbit