Anti-trimethyl-Histone H3 (Lys9) Antibody

Histone H3 is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

~17 kDa

BSA-conjugated, synthetic peptide containing the sequence …AR[me3K]S… in which me3K corresponds to trimethyl-lysine 9 of human Histone H3.

Epitope: Trimethyl Lys9

Anti-trimethyl-Histone H3 (Lys9) Antibody is a Rabbit Polyclonal Antibody for detection of trimethyl-Histone H3 (Lys9) also known as H3K9me3, Histone H3 (tri methyl K9) & has been validated in DB, PIA & WB.

Peptide Inhibition Analysis:
10 μM of a Histone H3 peptide containing trimethyl-lysine 9 abolished detection of Histone H3 by anti-trimethyl Histone H3 (Lys9) in western blot analysis of HeLa acid extracts (Figure B, lane 4). Slight signal reduction was observed with 10 μM of a Histone H3 peptide containing dimethyl-lysine 9.

Peptide Dot Blot Analysis:
A 1:2000 dilution of a previous lot was incubated with serial dilutions of Histone H3 peptides containing various modifications spotted onto PVDF. Slight cross-reactivity with Histone H3 peptide containing dimethyl-lysine 9 was detected.

Beadlyte Histone Peptide Assay:
1:2000-1:6000 dilutions of a previous lot were incubated with Histone H3 peptides containing various modifications conjugated to Luminex microspheres. Slight cross-reactivity with Histone H3 peptides containing dimethyl-lysine 9 and trimethyl-lysine 27 was detected.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

A broad species cross-reactivity is expected.

Recognizes Histone H3 containing trimethyl-lysine 9 and, to a lesser extent, dimethyl-lysine 9. MW ~17 kDa.

Immunodepleted Serum

Rabbit polyclonal antiserum IgG in buffer containing 0.05% sodium azide before the addition of 30% glycerol.

Stable for 1 year at -20ºC from date of receipt.

Control
Acid extracted proteins from HeLa cells.

Evaluated by western blot in acid-extracted proteins from HeLa cells.

Western Blot Analysis:
1:500-1:5000 dilution of this antibody detected methylated Histone H3 (Lys9) in acid-extracted proteins from HeLa cells. The antibody did not detect unmethylated recombinant Histone H3 (Catalog # 14-411).

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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