07-690
Anti-Histone H3 Antibody, CT, pan
serum, Upstate®
동의어(들):
H3, Histone H3, H3 histone family, member T, histone 3, H3, histone cluster 3, H3
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human, mouse, yeast, rat, chicken, Saccharomyces cerevisiae
Application:
ChIP
western blot
western blot
Technique(s):
ChIP: suitable
western blot: suitable
western blot: suitable
Citations:
274
Uniprot accession no.:
제품 이름
Anti-Histone H3 Antibody, CT, pan, serum, Upstate®
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse, yeast, rat, chicken, Saccharomyces cerevisiae
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... H3C1(8350)
mouse ... H3C1(360198)
rat ... H3C1(679994)
Analysis Note
Routinely evaluated by immunoblot on acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.
Western Blot Analysis:
1:25,000-1:50,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.
Western Blot Analysis:
1:25,000-1:50,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.
Application
Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate chromatin from yeast.
This antibody has been reported by an independent laboratory to immunoprecipitate chromatin from yeast.
Use Anti-Histone H3 Antibody, CT, pan (rabbit polyclonal antibody) validated in ChIP, WB to detect Histone H3 also known as H3, Histone H3, H3 histone family member T, histone 3 H3, histone cluster 3 H3.
Biochem/physiol Actions
Broad species cross-reactivity expected due to sequence homology.
Recognizes C-Terminal region of Histone H3, Mr 17 kDa
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Approx. 17 kDa
Immunogen
KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Rabbit antiserum diluted 1:2 in storage buffer (0.02 M Phosphate, 0.25 M NaCl, 0.1% sodium azide) before the addition of glycerol to 30%.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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저장 등급
10 - Combustible liquids
wgk
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
A novel domain in Set2 mediates RNA polymerase II interaction and couples histone H3 K36 methylation with transcript elongation.
Kizer, Kelby O, et al.
Molecular and cellular biology, 25, 3305-3316 (2005)
Reduced hepatic stellate cell expression of Kruppel-like factor 6 tumor suppressor isoforms amplifies fibrosis during acute and chronic rodent liver injury.
Ghiassi-Nejad, Z; Hernandez-Gea, V; Woodrell, C; Lang, UE; Dumic, K; Kwong, A; Friedman, SL
Hepatology null
Histone modifications on the adrenergic induction of type II deiodinase in rat pinealocytes.
Chik, CL; Price, DM; Ho, AK
Molecular and cellular endocrinology null
Neuronal cell depolarization induces intragenic chromatin modifications affecting NCAM alternative splicing.
Schor, IE; Rascovan, N; Pelisch, F; Allo, M; Kornblihtt, AR
Proceedings of the National Academy of Sciences of the USA null
Evelina Y Basenko et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(46), E6339-E6348 (2015-11-19)
H3K9 methylation directs heterochromatin formation by recruiting multiple heterochromatin protein 1 (HP1)-containing complexes that deacetylate histones and methylate cytosine bases in DNA. In Neurospora crassa, a single H3K9 methyltransferase complex, called the DIM-5,-7,-9, CUL4, DDB1 Complex (DCDC), is required for
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| 07-690 | 04053252582547 |
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