Anti-Wnt3A Antibody

The Wnt family of consists of ligands involved in cell development, fate determination, organogenesis, and oncogenesis. Information on Wnt signaling has been inferred from genetic analysis of wingless (wg) signaling in Drosophila. The Wnt signaling cascade initiates at the cell membrane when Wnt interacts with its receptor, a member of the Frizzled (Fz) transmembrane receptor family and lipoprotein receptor–related proteins 5 and 6 (LRP-5/6). This interaction relays the signal into the interior of the cell where disheveled (dsh) is brought to the membrane and gets phosphorylated/activated. Dsh promotes the inactivation of GSK3 kinase activity, thus preventing the phosphorylation of β-Catenin and allowing it to accumulate as it avoids ubiquination and subsequent proteosome degradation. Stable β-Catenin accumulates in the cytoplasm where it interacts with lymphoid enhancer factor/T cell factor (LEF/TCF) and translocates into the nucleus. This results in activation of TCF-responsive genes which are thought to play key roles in development and cancer. Negative regulators of the pathway may include axin and the tumor suppressor APC.

~40 kDa

Epitope: Central

Synthetic KLH-conjugated peptide directed against the central region of human, mouse, and rat Wnt3A.

Anti-Wnt3A Antibody is an antibody against Wnt3A for use in WB, IH(P).

Research Category
Signaling

Research Sub Category
Developmental Signaling

Recognizes Wnt3A

Affinity purified

Affinity purified rabbit polyclonal in storage buffer containing PBS and 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

routinely evaluated by immunoblot on rat brain lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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