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Merck

09-864

Anti-JHDM1B Antibody

from rabbit, purified by affinity chromatography

동의어(들):

JMJD, lysine (K)-specific demethylase 2B, F-box protein FBL10, [Histone-H3]-lysine-36 demethylase 1B, JEMMA (Jumonji domain, EMSY-interactor, methyltransferase motif) protein, F-box and leucine-rich repeat protein 10, protein containing CXXC domain 2, ju

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, WB
Citations:
30

주요 문서

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기술 서비스
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도움 문의

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human, rat

species reactivity (predicted by homology)

chimpanzee (based on 100% sequence homology), rabbit (based on 100% sequence homology), porcine (based on 100% sequence homology), horse (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable, western blot: suitable

NCBI accession no.

NP_115979

UniProt accession no.

Q8NHM5

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... KDM2B(84678)

관련 카테고리

General description

JmjC domain-containing histone demethylation protein 1B, also known as Lysine-specific demethylase 2B, is a nuclear enzyme encoded by the JHDM1B gene (also known as KDM2B) in human. JHDM1A/KDM2B exhibits H3K36-specific demethylase activity with a preference for H3K36me2 over H3K36me and H3K36me3 (PMID 16362057). JHDM1A/KDM2B is also reported to negatively regulate cell proliferation by targeting rDNA and thereby repressing the transcription of rRNA in a JmjC-domain-dependent manner (PMID 17994099).
~165 kDa observed. An uncharacterized lower molecular weight band may be observed in some lysates.

Immunogen

KLH-conjugated linear peptide corresponding to human JHDM1B.

Application

Anti-JHDM1B Antibody is a rabbit polyclonal antibody for detection of JHDM1B also known as lysine (K)-specific demethylase 2B, F-box protein FBL10, [Histone-H3]-lysine-36 demethylase 1B & has been validated in WB, ICC.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected JHDM1B in NIH/3T3 and HeLa cells.
Immunocytochemistry Analysis: 2 µg/mL from a representative lot detected JHDM1B in A431 cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa nuclear extract
Evaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 1 µg/mL of this antibody detected JHDM1B on 10 µg of HeLa nuclear extract.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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저장 등급

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Manabu Ozawa et al.
Biology of reproduction, 94(4), 92-92 (2016-03-18)
The F-box and leucine-rich repeat protein 10 (Fbxl10) gene encodes a protein that catalyzes demethylation of H3K4 and H3K36. In this study, we show the important roles of FBXL10 as a histone demethylase in sustainable sperm production using mice in
Nefeli Zacharopoulou et al.
Cancer biology & therapy, 21(6), 533-540 (2020-03-17)
Recent studies revealed that the histone demethylase KDM2B regulates the epithelial markers E-Cadherin and ZO-1, the RhoA/B/C-small-GTPases and actin cytoskeleton organization, in DU-145 prostate- and HCT-116 colon-tumor cells. Here we addressed the role of KDM2B in the activation of Focal
Yan Kuang et al.
Cancer science, 112(1), 231-242 (2020-10-23)
MicroRNA let-7b is a potent tumor suppressor and targets crucial oncogenes. Previous studies have shown that let-7b expression is suppressed in ovarian cancer; however, the regulatory mechanisms of let-7b in ovarian cancer are still not well defined. The cellular role
Polycomb group and SCF ubiquitin ligases are found in a novel BCOR complex that is recruited to BCL6 targets.
Gearhart, MD; Corcoran, CM; Wamstad, JA; Bardwell, VJ
Molecular and cellular biology null
Nefeli Zacharopoulou et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 47(1), 368-377 (2018-05-18)
The epigenetic factor KDM2B is a histone demethylase expressed in various tumors. Recently, we have shown that KDM2B regulates actin cytoskeleton organization, small Rho GTPases signaling, cell-cell adhesion and migration of prostate tumor cells. In the present study, we addressed
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관련 콘텐츠

NPI Flyer- Epigenetics and Nuclear Function Feature

Historically chromatin was considered to contain only DNA, histones, non-histone proteins and transiently associated mRNAs. While this remains true, current data suggest that a variety of non-coding RNAs (e.g. long non-coding RNAs, enhancer RNAs and even miRNAs) also associate with chromatin and serve regulatory functions.

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