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Merck

17-295

Chromatin Immunoprecipitation (ChIP) Assay Kit

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using inexpensive protein A agarose beads.

동의어(들):

Agarose ChIP Kit, ChIP, Chromatin enrichment, chromatin immunoprecipitation

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12161503
NACRES:
NA.41
eCl@ss:
32161000
기술 서비스
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도움 문의
기술 서비스
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도움 문의

manufacturer/tradename

EZ-ChIP

technique(s)

immunoprecipitation (IP): suitable

shipped in

wet ice

Quality Level

관련 카테고리

General description

For use to enrich for specific regions of chromatin that are immunoprecipitated with a specific antibody for a chromatin protein. Works with all eukaryotic cell types. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR detection is recommended.

Packaging

Kit capacity: 22 Assays

Physical form

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).

Analysis Note

Routinely evaluated using anti-acetyl Histone H4 polyclonal antibody (06-866) to immunoprecipitate acetylated histone H4. Subsequent immunoblot analysis was done using the same antibody.

Other Notes

Protein A agarose/Salmon Sperm DNA (Cat.# 16-157)

All necessary buffers

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

저장 등급

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Epigenetic regulation of the endothelial nitric oxide synthase gene in persistent pulmonary hypertension of the newborn rat.
Xu, XF; Ma, XL; Shen, Z; Wu, XL; Cheng, F; Du, LZ
Journal of Hypertension null
Seed-based systematic discovery of specific transcription factor target genes.
Mrowka, R; Bluthgen, N; Fahling, M
FEBS Journal null
M Vignali et al.
The EMBO journal, 19(11), 2629-2640 (2000-06-03)
We analyzed the targeting of histone acetyltransferase (HAT) complexes by DNA-binding activators during transcriptional activation and the resulting distribution of acetylated histones. An in vitro competition assay was developed to acetylate and transcribe a nucleosomal array template in the presence
A novel cis-acting element in Her2 promoter regulated by Stat3 in mammary cancer cells
Qian, Lu, et al
Biochemical and biophysical research communications null
Regulation of human papillomavirus type 31 gene expression during the differentiation-dependent life cycle through histone modifications and transcription factor binding.
Tonia R Wooldridge, Laimonis A Laimins
Virology null

관련 콘텐츠

Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.

단백질 및 핵산 상호작용 시약과 단백질-RNA, 단백질-DNA, 단백질-단백질 상호작용 및 관련 애플리케이션 투자용 관련 자료입니다.

The 3rd edition of An Introduction to Antibodies and Their Applications provides a concise overview of some of the key features for the use of antibodies and immunochemical techniques in biological research. This handy reference guide supplements the techniques described in literature, recorded in general laboratory procedures, and described on individual product data sheets. Antibody design, development, and production are our expertise. Stringent validation of our antibodies is only one component of a comprehensive process we undertake to provide the antibodies most cited by the research community (see section Antibody Quality on page 2 for an in-depth look at our expertise).

"Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms instead of by alterations in DNA sequence. These changes can be cell- or tissue-specific, and can be passed on to multiple generations. Epigenetic regulation enriches DNAbased information, allowing a cell to vary its response across diverse biological and environmental contexts. Although epigenetic mechanisms are primarily centered in the nucleus, these mechanisms can be induced by environmental signals such as hormones, nutrients, stress, and cellular damage, pointing to the involvement of cytoplasmic and extracellular factors in epigenetic regulation."

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17-29508436037124679

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