324715
Lys-C Protease
Precise cleavage at lysine residues, suitable for mass spectrometry, from Lysobacter enzymogenes
동의어(들):
Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
UNSPSC Code:
12352202
MDL number:
NACRES:
NA.54
Specific activity:
≥200 units/mg protein
Assay:
≥90% (SDS-PAGE)
제품 이름
Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes,
grade
Proteomics Grade
assay
≥90% (SDS-PAGE)
form
lyophilized
specific activity
≥200 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated (hygroscopic)
shipped in
ambient
storage temp.
2-8°C
Quality Level
General description
Native endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
Physical form
Lyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
Reconstitute in 50 µl of distilled H₂O.
Other Notes
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Tos-Gly-Pro-Lys-pNA per min at 25°C, pH 7.7.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
S Zhang et al.
The Journal of biological chemistry, 271(52), 33575-33579 (1996-12-27)
While multiple G protein beta and gamma subunit isoforms have been identified, the implications of this potential diversity of betagamma heterodimers for signaling through betagamma-regulated effector pathways remains unclear. Furthermore the molecular mechanism(s) by which the betagamma complex modulates diverse
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| 324715-5UGCN | 04055977215960 |
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