Anti-Aggrecan Antibody

Aggregating chondroitin sulfate proteoglycan or aggrecan, is one of the most studied proteoglycans. Many individual monomers of aggrecan bind to hyaluronic acid to form an aggregate, the monomer is known as aggrecan. The aggregates are comprised of up to 100 monomers attached to a single chain of hyaluronic acid. The high content of negatively charged polysaccharide chains contributes to the osmotic swelling of cartilage and in combination with intact collagen fibers results in articular cartilage resistance to compressive forces and high tensile strength. Aggrecan is abundant in articular cartilage and compromises 10% of its dry weight.

GST fusion protein containing amino acids 1177-1326 of mouse aggrecan.

Anti-Aggrecan Antibody is an antibody against Aggrecan for use in IF, IH(P), IP & WB.

Immunohistochemistry(Paraffin):
A previous lot of this antibody was used at 10 μg/mL. See note under Protocol regarding triton X-100 treatments.

Immunoprecipitation:
A previous lot of this antibody was used at 5 μg/ 500 μL of cell membrane extract in PBS with inhibitors and >0.5% triton X-100.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

AB1031 is specific for mouse aggrecan; other species have not been tested.

Expected to cross react with Human and rat.

ImmunoAffinity Purified

Rabbit polyclonal antigen affinity purified antibody in PBS. Anti-GST has not been adsorbed. Liquid.
Note: Aggrecan is present in articular cartilage, brain, heart, and other tissues.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
Cartilage, neural tube, and brain tissue.

Routinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot Analysis:
1:500 dilution of this lot detected AGGRECAN on 10 μg of Mouse Brain lysates.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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