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Merck

AB5539

Anti-Neurofilament H (200 kDa) Antibody

CHEMICON®, chicken polyclonal

동의어(들):

NF-H

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
rat, pig, bovine, feline, human, mouse
Application:
ICC, IHC (p), WB
Citations:
192
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제품 이름

Anti-Neurofilament H Antibody, Chemicon®, from chicken

biological source

chicken

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, pig, bovine, feline, human, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

Gene Information

bovine ... Nefh(528842)
cat ... Nefh(101087272)
human ... NEFH(4744)
mouse ... Nefh(380684)
pig ... Nefh(100156492)
rat ... Nefh(24587)

General description

200 kDa

Immunogen

Purified bovine NF-H.

Application

Anti-Neurofilament H Antibody is an antibody against Neurofilament for use in IC, IH, IH(P) & WB.
Western blot: 1:1,000-1:10,000 using ECL. Recognizes a band of 200-220 kDa.

Immunohistochemistry: 1:1,000-1:10,000 on frozen or paraffin embedded tissue sections. Suggested fixative is 4% paraformaldehyde.

Immunocytochemistry: 1:1,000-1:10,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes Neurofilament-Heavy (NF-H). The antibody stains sharply defined cytoplasmic filaments in neuronal cell bodies and especially axons.

Physical form

Format: Purified
Purified chicken IgY in PBS containing 5mM sodium azide.

Analysis Note

Control
Positive control: brain.

Negative control: kidney, liver.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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저장 등급

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Computation identifies structural features that govern neuronal firing properties in slowly adapting touch receptors.
Lesniak, DR; Marshall, KL; Wellnitz, SA; Jenkins, BA; Baba, Y; Rasband, MN; Gerling, GJ; Lumpkin, EA
eLife null
Lucas M Viana et al.
Hearing research, 327, 78-88 (2015-05-24)
Recent animal work has suggested that cochlear synapses are more vulnerable than hair cells in both noise-induced and age-related hearing loss. This synaptopathy is invisible in conventional histopathological analysis, because cochlear nerve cell bodies in the spiral ganglion survive for
Katarzyna Dover et al.
Nature communications, 7, 12895-12895 (2016-09-27)
Neurons in vertebrate central nervous systems initiate and conduct sodium action potentials in distinct subcellular compartments that differ architecturally and electrically. Here, we report several unanticipated passive and active properties of the cerebellar granule cell's unmyelinated axon. Whereas spike initiation
Juan Miguel Jimenez-Andrade et al.
Bone, 46(2), 306-313 (2009-09-22)
Although musculoskeletal pain is one of the most common causes of chronic pain and physical disability in both developing and developed countries, relatively little is known about the nerve fibers and mechanisms that drive skeletal pain. Small diameter sensory nerve
Zu-Lin Chen et al.
Developmental biology, 327(1), 158-168 (2009-01-03)
Laminins have dramatic and varied actions on neurons in vitro. However, their in vivo function in brain development is not clear. Here we show that knockout of laminin gamma1 in the cerebral cortex leads to defects in neuritogenesis and neuronal

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SKUGTIN
AB553904053252506338

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