제품 이름
Anti-Neurofilament H Antibody, Chemicon®, from chicken
biological source
chicken
Quality Segment
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, pig, bovine, feline, human, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
bovine ... Nefh(528842)
cat ... Nefh(101087272)
human ... NEFH(4744)
mouse ... Nefh(380684)
pig ... Nefh(100156492)
rat ... Nefh(24587)
General description
200 kDa
Immunogen
Purified bovine NF-H.
Application
Anti-Neurofilament H Antibody is an antibody against Neurofilament for use in IC, IH, IH(P) & WB.
Western blot: 1:1,000-1:10,000 using ECL. Recognizes a band of 200-220 kDa.
Immunohistochemistry: 1:1,000-1:10,000 on frozen or paraffin embedded tissue sections. Suggested fixative is 4% paraformaldehyde.
Immunocytochemistry: 1:1,000-1:10,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:1,000-1:10,000 on frozen or paraffin embedded tissue sections. Suggested fixative is 4% paraformaldehyde.
Immunocytochemistry: 1:1,000-1:10,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Recognizes Neurofilament-Heavy (NF-H). The antibody stains sharply defined cytoplasmic filaments in neuronal cell bodies and especially axons.
Physical form
Format: Purified
Purified chicken IgY in PBS containing 5mM sodium azide.
Analysis Note
Control
Positive control: brain.
Negative control: kidney, liver.
Positive control: brain.
Negative control: kidney, liver.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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저장 등급
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
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