CC052
Human Collagen Type II
liquid, 1 mg/mL, suitable for cell culture, used for gel formation
동의어(들):
Collagen Type II, Human Collagen
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제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75
Biological source:
human
Form:
liquid, solution
Technique(s):
cell culture | mammalian: suitable
Concentration:
1 mg/mL
Assay:
90% (Human collagen type II)
제품 이름
Human Collagen Type II, 1 mg/mL, solution, BioReagent, suitable for cell culture
biological source
human
Quality Segment
assay
90% (Human collagen type II)
form
liquid, solution
manufacturer/tradename
Chemicon®
concentration
1 mg/mL
technique(s)
cell culture | mammalian: suitable
impurities
<0.5% Non-collagen proteins, <1% Human collagen type IV, <1% Human collagen type V, <10% Human collagen type I, <10% Human collagen type III
input
sample type pancreatic stem cell(s)
sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type induced pluripotent stem cell(s)
sample type mesenchymal stem cell(s)
sample type hematopoietic stem cell(s)
sample type neural stem cell(s)
solubility
water: soluble at 20 °C
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... COL2A1(1280)
General description
Type II collagen, a fibrillar collagen, is the main component of hyaline cartilage and constitutes 95% of the collagens and approximately 60% of cartilage’s dry weight. It is a homotrimer protein encoded by the gene COL2A1, mapped to human chromosome 12q13. Type two Collagen is also found in the nucleous pulposo of the intervertebral disc and the retina, sclera, and lens of the eye. Type IIA, is localized mainly to the vitreous body of the eye. Type IIB, is preferentially produced in adult cartilage tissue. Collagen type II was purified by using differential salt precipitation, alcohol precipitation and DEAE chromatography. Multiple purification steps provide final preparations of collagen types in the native triple-helical form, free of any non-collagenous protein. Contamination of other collagen types in a standard preparation is lower than five percent.
Application
Human Collagen Type II has been used:
- as an extracellular matrix (ECM) protein in microarray analysis, to coat on nitrocellulose film slides for probing and detection experiments
- to study the role of Col2a1a in the neural crest during early eye development in zebrafish model of Stickler syndrome
- as a calibration standard in the quantification of collagen II levels in scaffolds using an enzyme-linked immunosorbent assay (ELISA)-based assay
Biochem/physiol Actions
Type II collagen is commonly co-assembled with collagen XI, forming a complex that is covalently crosslinked to collagen IX and interacts with small leucine-rich proteoglycans. Collagen 2 imparts stability and strength to the tissue and maintains integrity and resilience under stress. Cleavage of Type II collagen occurs mainly through the action of matrix metalloproteinase collagenases, leading to the generation of well-known biomarkers such as the C-terminal telopeptide of Type II collagen and C2C. Mutations in type II collagen cause chondrodysplasia, leading to premature osteoarthritis.
Physical form
Purified protein. Liquid in 0.5M acetic acid. No preservative.
Preparation Note
Aliquot and freeze. Maintain at -20°C up to 12 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.
저장 등급
12 - Non Combustible Liquids
WGK
WGK 1
인화점 (F)
Not applicable
인화점 (°C)
Not applicable