Milli-Mark Anti-CD4 -FITC Antibody, clone MT310

55 kDa (Leong, 1999)

CD4 is a 55 kDa glycoprotein with four immunoglobulin-like external domains, a transmembrane domain and a highly conserved intracellular domain (Leong, 1999). CD4 acts as a coreceptor in MHC class II-restricted antigen-induced T-cell activation. Further, CD4 has a role in the regulation of T-B lymphocyte adhesion in the absence of antigen recognition, and in thymic differentiation (Piatier-Tonneau, 2002). CD4 is expressed on thymocyte subsets, it marks helper/inducer T cells, and it is expressed in 55-65% of mature peripheral T cells. Subpopulations of suppressor or cytotoxic T cells can be identified among CD4-positive T cells. CD4 is also expressed on monocytes/macrophages, Langerhans cells and other dendritic cells. CD4 is not expressed on B cells. Anti-CD4, MT310, was included in the Second International Workshop and Conference on Human Leucocyte Differentiation Antigens, and studies by a number of laboratories confirmed its reactivity with the CD4 antigen (Miedema, 1986).

Milli-Mark Anti-CD4 -FITC Antibody, clone MT310 is an antibody against CD4 for use in FC.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Antibody recognizes Human CD4 antigen.

Protein A purified

Purified Mouse monoclonal IgG1 kappa conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Control
Human Lymphocytes

Evaluated by flow cytometry using human lymphocytes.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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