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Merck

IPFL00010

Immobilon® -FL PVDF Membrane

1 roll, 27 cm x 3.75 m, 0.45 µm pore size, transfer membrane with low background fluorescence

동의어(들):

Western blotting membrane, blotting membrane, transfer membrane

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric
Pore size:
0.45 μm pore size
Compatibility:
for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby

주요 문서

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제품 이름

Immobilon®-FL PVDF Membrane, 1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

material

PVDF membrane, plain filter, white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

filter L × W

27 cm × 3.75 m

pore size

0.45 μm pore size

capacity

155 μg/cm2 adsorption capacity (insulin), 205 μg/cm2 adsorption capacity (BSA), 300 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby

detection method

chemiluminescent, colorimetric, fluorometric

shipped in

ambient

Quality Level

400

관련 카테고리

Application

Immobilon-FL PVDF Membrane has been used in western blotting.

Features and Benefits

Immobilon-FL Membrane:
  • The first transfer membrane optimized for fluorescence applications; Extremely low background enhances the sensitivity of all fluorescence detection protocols
  • Compatible with all commonly used fluorescent probes at a wide range of excitation/emission wavelengths
  • Best for multiplexing and chemifluorescence detections
  • compatible with standard blocking agents and buffers

General description

The Immobilon®-FL transfer membrane is a polyvinylidene fluoride (PVDF) microporous membrane with a nominal pore size of 0.45 μm. It is designed for binding proteins transferred from a variety of gel matrices. The Immobilon®-FL transfer membrane is hydrophobic and offers a uniformly controlled pore structure with a high binding capacity for biomolecules. The membrane offers optimal blotting for proteins with molecular weights greater than 20,000. The Immobilon®-FL membrane displays very low autofluorescence across a wide range of excitation/emission wavelengths. This property makes it ideal for blotting applications involving fluorescence-based immunodetection. Immobilon®-FL membrane can also be used for standard chemiluminescent or chromogenic detection.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

S A Misek et al.
Oncogene, 39(7), 1466-1483 (2019-10-30)
Over half of cutaneous melanoma tumors have BRAFV600E/K mutations. Acquired resistance to BRAF inhibitors (BRAFi) remains a major hurdle in attaining durable therapeutic responses. In this study we demonstrate that ~50-60% of melanoma cell lines with vemurafenib resistance acquired in
Guangchuan Wang et al.
Biological research, 52(1), 60-60 (2019-12-19)
Recent studies have confirmed that RASAL1 has an antitumor effect in many cancers, but its functional role and the molecular mechanism underlying in colon cancer has not been investigated. We collected human colon cancer tissues and adjacent non-tumor tissues, human
David J Ferland et al.
PloS one, 15(2), e0229251-e0229251 (2020-02-25)
Since chemerin's identification as an adipokine, it has been associated with a number of human diseases including diabetes and obesity. However, the basic scientific foundation for these clinical determinations is still lacking. Fibroblastic mouse 3T3 cells are unable to develop
Enrique Calvo et al.
Science advances, 6(26), eaba7509-eaba7509 (2020-07-09)
Mitochondrial respiratory complexes assemble into supercomplexes (SC). Q-respirasome (III2 + IV) requires the supercomplex assembly factor (SCAF1) protein. The role of this factor in the N-respirasome (I + III2 + IV) and the physiological role of SCs are controversial. Here
Daniel Del Valle Morales et al.
Nucleic acids research, 48(7), 3806-3815 (2020-01-31)
Cap homeostasis is the cyclical process of decapping and recapping that maintains the translation and stability of a subset of the transcriptome. Previous work showed levels of some recapping targets decline following transient expression of an inactive form of RNMT
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프로토콜

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Stripping and Reprobing Western Blotting Membranes

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

관련 콘텐츠

Protein Research Guide

Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.

Immobilon®-E Transfer Membrane User Guide
Low Background Membrane for Fluorescent Protein Detection in Western Blotting
Immobilon® Block Noise Cancelling Reagents
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국제 무역 품목 번호

SKUGTIN
IPFL0001004053252669439

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