biological source
mouse
Quality Segment
conjugate
CY3 conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
113-1, monoclonal
species reactivity
human (mitochondria protein)
should not react with
rat (mitochondria protein), mouse (mitochondria protein)
technique(s)
immunocytochemistry: suitable
isotype
IgG1
shipped in
wet ice
target post-translational modification
unmodified
General description
60 kDa calculated
This antibody recognizes a 60 kDa nonglycosylated protein component of mitochondria found in human cells. It can be used to stain mitochondria in cell, tissue or biochemical preparations and can be used as a marker of the mitochondria in subcellular fractions. MAB1273C3 recognizes a 65 kD protein by immunoprecipitation and produces the stringy, "spaghetti-like", staining patten in the cytoplasm of human cells.
Immunogen
Surface of intact human mitochondria
Application
Detect Mitochondrial Marker using this mouse monoclonal antibody, Anti-Mitochondria Antibody, clone 113-1 & Cy3 Conjugate validated for use in ICC.
Evaluated by Immunocytochemistry in human adipose mesenchymal stem cells (SCC038).
Immunocytochemsitry Analysis: A 1:50 dilution of this antibody detected mitrochondria in human adipose mesenchymal stem cells (SCC038).
Immunocytochemsitry Analysis: A 1:50 dilution of this antibody detected mitrochondria in human adipose mesenchymal stem cells (SCC038).
Research Category
Stem Cell Research
Cell Structure
Stem Cell Research
Cell Structure
Research Sub Category
Developmental Neuroscience
Organelle & Cell Markers
Developmental Neuroscience
Organelle & Cell Markers
Biochem/physiol Actions
This antibody recognizes human. Antibody is not reactive with mouse or rodent.
Physical form
Protein G Purified
Purified mouse monoclonal IgG1 conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.
Preparation Note
Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
Neonatal human fibroblasts cells (SCC058).
Neonatal human fibroblasts cells (SCC058).
Evaluated by Immunocytochemistry in neonatal human fibroblasts cells (SCC058).
Immunocytochemsitry Analysis: A 1:50 dilution of this antibody detected mitrochondria in neonatal human fibroblast cells (SCC058).
Immunocytochemsitry Analysis: A 1:50 dilution of this antibody detected mitrochondria in neonatal human fibroblast cells (SCC058).
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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저장 등급
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
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