Anti-Human B cells (CD19) Antibody, clone FMC63, Phycoerythrin conjugated

BIOCHEMISTRY:

p I = 7.0

Raji cells

Detect CD19 using this Anti-Human B cells (CD19) Antibody, clone FMC63, Phycoerythrin conjugated validated for use in FC.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This antibody is useful for enumerating normal B lymphocytes and typing B cell malignancies by flow cytometry.

SUGGESTED USAGE

Flow cytometry - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E(6) peripheral blood mononuclear cells (PBMC) in 100 μl buffer.

This specificity of this antibody is equivalent to that of CD19 monoclonal antibodies (Zola et al., 1988, 1989), based on comparative studies, antigen recognition and positive reaction with a CD19 transfected cell line. It detects a 96 kD single chain glycoprotein on the surface of human B lineage cells, including pre-B cells. B cells can be detected in peripheral blood and tissues. The antibody reacts with most B cell malignancies including chronic lymphocytic leukemia and most lymphomas, but not multiple myeloma.

Cell reactivity:

Normal:Stains all B lymphocytes in peripheral blood and spleen. Negative on granulocytes, monocytes, platelets, erythrocytes and T lymphocytes.

Clinical:

B cell chronic lymphocytic leukemia (B-CLL) +

Pro lymphocytic leukemia (PLL +

Hairy cell leukemia (HCL) +

Common acute lymphoblastic leukemia (CALL) +

Pre-B acute lymphoblastic leukemia (pre-B-ALL) +

NULL-acute lymphoblastic leukemia (NULL-ALL) +

T-acute lymphoblastic leukemia (T-ALL) -

T-common lymphocytic leukemia (T-CLL -

Purified from mouse ascitic fluid. R-Phycoerythrin conjugated. The antibody is supplied in 1.0ml PBS 0.01M, Sodium Azide 0.1%, pH 7.1, 0.2% BSA. The characteristics of each lot are tested by electrophoresis and flow cytometry.

Store at 2 to 8°C, for up to 6 months. DO NOT FREEZE. Protect from light.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Replaces: CBL141P

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.