MAB1951
Anti-Integrin beta 1 (CD29) Antibody
CHEMICON®, mouse monoclonal, P4G11
동의어(들):
CD29
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크기 선택
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제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
P4G11, monoclonal
Species reactivity:
monkey, rabbit, human
Application:
ELISA, FACS, ICC, IHC (p), IP, cell culture
Citations:
15
제품 이름
Anti-Integrin β1 Antibody, clone P4G11, clone P4G11, Chemicon®, from mouse
biological source
mouse
Quality Segment
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
P4G11, monoclonal
species reactivity
monkey, rabbit, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, cell culture | mammalian: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ITGB1(3688)
Immunogen
Human IL-1beta activated endothelial cells
Application
Anti-Integrin β1 Antibody, clone P4G11 is an antibody against Integrin β1 for use in ELISA, FC, IC, IH, IH(P), IP, FUNC, CULT.
Immunohistochemistry: for use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and 4% PFA paraffin embedded {Qin et al 2003 1:500, citrate HEIR treated}
Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.
Traditional formalin fixation is not recommended.
Immunocytochemistry: 2 ug/mL (for use on numerous cell lines as long as Ca2+ is present)
Immunoprecipitation: 5 μg/400 μL lysate (0.5% triton PBS plus Ca++)
FACS: 2-5μg per 10e6 cells, in the presence of Ca++.
Stimulation of cells: 2-10 μg/ml per 10e6 cells. Binding buffer must contain 1mM Ca++.
ELISA
Optimal working dilutions must be determined by end user.
Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.
Traditional formalin fixation is not recommended.
Immunocytochemistry: 2 ug/mL (for use on numerous cell lines as long as Ca2+ is present)
Immunoprecipitation: 5 μg/400 μL lysate (0.5% triton PBS plus Ca++)
FACS: 2-5μg per 10e6 cells, in the presence of Ca++.
Stimulation of cells: 2-10 μg/ml per 10e6 cells. Binding buffer must contain 1mM Ca++.
ELISA
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
Biochem/physiol Actions
Reacts with calcium-dependent epitopes on the integrin β1 subunit. When bound to β1, P4G11, activates β1 dependent binding to extra cellular matrix substrates.
Physical form
Format: Purified
Protein A Purified immunoglobulin in 0.02M PBS, 0.25M NaCl, pH 7.6, with 0.1% NaN3.
Protein A purified
Preparation Note
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Tonsil, human skin tissue
Tonsil, human skin tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
저장 등급
12 - Non Combustible Liquids
시험 성적서(COA)
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