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Merck

MAB4304

Anti-Stage-Specific Embryonic Antigen-4 Antibody, clone MC-813-70

clone MC-813-70, Chemicon®, from mouse

동의어(들):

SSEA-4

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
MC-813-70, monoclonal
Species reactivity:
human, mouse
Application:
ELISA, FACS, IF, IHC
Citations:
216

주요 문서

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기술 서비스
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도움 문의
기술 서비스
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도움 문의

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MC-813-70, monoclonal

species reactivity

human, mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, flow cytometry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable

input

sample type: mouse embryonic stem cell(s)
sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

isotype

IgG3

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

관련 카테고리

General description

The cell-surface markers used routinely to identify undifferentiated human embryonic stem cells (hESCs) were initially characterized as markers for mESCs, mouse embryonic carcinomas (ECs), or human EC cells. Stage-specific embryonic antigen-3 (SSEA-3) and SSEA-4 are widely used as cell-surface markers to define both human and mouse ESCs. SSEA-3 and SSEA-4 are expressed on undifferentiated hESCs but not on undifferentiated mESCs.

Immunogen

Human embryonal carcinoma cell line 2102Ep

Application

Anti-Stage-Specific Embryonic Antigen-4 Antibody, clone MC-813-70 is an antibody against Stage-Specific Embryonic Antigen-4 for use in FC, ELISA, IF, IH.
Immunohistochemistry: A previous lot of this antibody was used in IH.

Immunofluorescence: A previous lot of this antibody was used in IF.

ELISA: A previous lot of this antibody was used in ELISA.

Flow Cytometry: A starting range of 10-20 µg/mL is suggested.

Optimal working dilutions must be determined by the end user.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Biochem/physiol Actions

This antibody reacts with the Stage-specific embryonic antigen-4 (SSEA-4) that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-4 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.

Physical form

Format: Purified
Protein A purified
Purified moust monoclonal IgG3 liquid in buffer containing 0.02 M PBS, 0.25 M NaCl, pH 7.6 with 0.1% Sodium Azide

Preparation Note

Stable for 1 year at from date of receipt.

Analysis Note

Control
Pluripotent human embryonic stem (ES) cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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저장 등급

10 - Combustible liquids

wgk

WGK 2

시험 성적서(COA)

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문서 라이브러리 방문

James A Byrne et al.
PloS one, 4(9), e7118-e7118 (2009-09-24)
The derivation of induced pluripotent stem cells (iPSCs) provides new possibilities for basic research and novel cell-based therapies. Limitations, however, include our current lack of understanding regarding the underlying mechanisms and the inefficiency of reprogramming. Here, we report identification and
Hidehito Saito et al.
Stem cells international, 2016, 8394960-8394960 (2016-04-09)
Induced pluripotent stem cells (iPSCs) derived from somatic cells of patients hold great promise for autologous cell therapies. One of the possible applications of iPSCs is to use them as a cell source for producing autologous lymphocytes for cell-based therapy
Lei Kang et al.
Stem cells and development, 25(7), 556-568 (2016-02-18)
Podocalyxin-like protein (PODXL) is a member of CD34 family proteins. It is the protein that carries many post-translational epitopes responsible for various pluripotent surface markers including TRA-1-60, TRA-1-81, GCTM2, GP200, and mAb84. However, PODXL has not attracted the attention of
Livia Eiselleova et al.
The International journal of developmental biology, 52(4), 353-363 (2008-04-17)
Various types of feeder cells have been adopted for the culture of human embryonic stem cells (hESCs) to improve their attachment and provide them with stemness-supporting factors. However, feeder cells differ in their capacity to support the growth of undifferentiated
Epigenetic characterization of the FMR1 gene and aberrant neurodevelopment in human induced pluripotent stem cell models of fragile X syndrome.
Sheridan, SD; Theriault, KM; Reis, SA; Zhou, F; Madison, JM; Daheron, L; Loring, JF; Haggarty, SJ
Testing null
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관련 콘텐츠

A Comparative Analysis of Human Embryonic Stem Cells Cultured in a Variety of Media Conditions
Human Stem Cell Systems

As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.

Efficient generation of transgene-free human and mouse iPS cells using a cell-permeant Tat-Cre protein
Accumax Dissociation Reagent for the Serial Passaging of Human Embryonic Stem Cells
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국제 무역 품목 번호

SKUGTIN
MAB430408436037123443

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