MAB5428
Anti-Retinal Pigment Epithelium 65 Antibody
clone 401.8B11.3D9, Chemicon®, from mouse
동의어(들):
RPE65
조직 및 계약 가격을 보려면 로그인를 클릭합니다.
크기 선택
제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
401.8B11.3D9, monoclonal
Application:
ELISA, IHC, IP, WB
Citations:
42
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
401.8B11.3D9, monoclonal
species reactivity
bovine, human, Xenopus, mouse
packaging
antibody small pack of 25 μg
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... RPE65(6121)
General description
~ 65 kDa
Immunogen
Bovine RPE microsomal membranes.
Application
Anti-Retinal Pigment Epithelium 65 Antibody detects level of Retinal Pigment Epithelium 65 & has been published & validated for use in ELISA, IH, IP & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Sensory & PNS
Sensory & PNS
Western blot: 1:5,000-1:20,000 on bovine RPE membranes using ECL. Suggested dilution buffer is TBS containing 10% calf serum, 0.25% T-20, 1M D-glucose with 10% glycerol. Suggested blocking buffer is TBS containing 2% BSA and 0.5% Tween 20. Preferred gel percentage is 10%.
Immunohistochemistry on frozen tissue sections: 1:250-1:500
Immunoprecipitation: 20 μg of antibody in a reaction volume of 500 μL.
Immunoaffinity purification
ELISA
Optimal working dilutions must be determined by end user.
Immunohistochemistry on frozen tissue sections: 1:250-1:500
Immunoprecipitation: 20 μg of antibody in a reaction volume of 500 μL.
Immunoaffinity purification
ELISA
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Reacts with Retinal Pigment Epithelium 65 (RPE65). On bovine RPE membranes the antibody recognizes a protein with a molecular weight of ~65 kDa.
Physical form
Format: Purified
Protein A purified
Purified immunoglobulin. Liquid in PBS. Contains no preservative.
Preparation Note
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Eye
Eye
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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당사의 제품 선택기 도구.을(를) 시도해 보세요.
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
저장 등급
12 - Non Combustible Liquids
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
L I Rachek et al.
Endocrinology, 148(1), 293-299 (2006-10-07)
A major characteristic of type 2 diabetes mellitus (T2DM) is insulin resistance in skeletal muscle. A growing body of evidence indicates that oxidative stress that results from increased production of reactive oxygen species and/or reactive nitrogen species leads to insulin
Xiao Yang et al.
eLife, 9 (2020-04-03)
The choroid, which provides vascular supply to the outer retina, demonstrates progressive degeneration in aging and age-related macular degeneration (AMD). However mechanisms that maintain or compromise choroidal homeostasis are obscure. We discovered that the ablation of choroidal macrophages via CSF1R
Manabu Hirasawa et al.
The Journal of biological chemistry, 291(14), 7373-7385 (2016-02-04)
Choroidal neovascularization (CNV) is a pathogenic process of age-related macular degeneration, a vision-threatening disease. The retinal pigment epithelium and macrophages both influence CNV development. However, the underlying mechanisms remain obscure. Here, we focus on Angptl2 (angiopoietin-like protein 2), a cytokine
Glenn Yiu et al.
Molecular therapy. Methods & clinical development, 16, 179-191 (2020-02-15)
Retinal gene therapy using adeno-associated viruses (AAVs) is constrained by the mode of viral vector delivery. Intravitreal AAV injections are impeded by the internal limiting membrane barrier, while subretinal injections require invasive surgery and produce a limited region of therapeutic
Retinoid uptake, processing, and secretion in human iPS-RPE support the visual cycle.
Mu?iz, A; Greene, WA; Plamper, ML; Choi, JH; Johnson, AJ; Tsin, AT; Wang, HC
Investigative Ophthalmology & Visual Science null
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| MAB5428 | 04053252664472 |
| MAB5428-25UG | 04054839337574 |
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