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PICM0RG50

Millicell® 6 well standing inserts

pore size 0.4 μm, diam. 30 mm, transparent PTFE membrane, hydrophilic, H 5 mm, sterile

동의어(들):

Millicell Cell Culture Insert, 30 mm, hydrophilic PTFE, 0.4 µm

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
41104923
NACRES:
NB.22
eCl@ss:
32011202
Material:
transparent PTFE membrane, polystyrene housing (5 mm height)
Pore size:
0.4 μm
Sterility:
ethylene oxide treated, sterile
Binding type:
low binding surface
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도움 문의

material

transparent PTFE membrane, polystyrene housing (5 mm height)

Quality Level

400

sterility

ethylene oxide treated, sterile

feature

hydrophilic

manufacturer/tradename

Millicell®

packaging

pkg of Individually blister packaged

parameter

50 °C max. temp.

technique(s)

cell attachment: suitable, cell culture | mammalian: suitable, cell differentiation: suitable

H

5 mm

diam.

30 mm

size

6 wells

surface area

4.2 cm2

color

transparent membrane, when wetted

matrix

Biopore™

pore size

0.4 μm

binding type

low binding surface

detection method

fluorometric

shipped in

ambient

storage temp.

10-30°C

General description

With Millicell inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell inserts make it possible to study both sides of the cell monolayer.

Application

  • Cell Attachment, 3D Cell Culture, Cell Growth, Cell Differentiation , Immunocytochemistry
  • Biopore Membrane (hydrophilic PTFE) is ideal for low protein-binding, live cell viewing, and immunofluorescent applications

Features and Benefits

  • For high cell viability and superior study of three dimensional explant structure
  • Lower height allows them to fit inside a standard petri dish
  • The Biopore (PTFE) membrane provides high viability—for as long as 40 days—and excellent trans-membrane oxygen transport
  • The membrane is optically clear and optimized for long-term organotypic explant maintenance

Preparation Note

To ensure proper wetting of the membrane from the basolateral side and to mitigate a chance of bubble formation:
  1. Add assay solution or media to the receiver well: 2 mL for 6 well format orequivalent dimensions.
  2. With forceps, place the insert into the receiver well at a 30°-45° angle togradually wet it out from the basolateral side.
  3. Once edges start to wet out, gently lower the insert until it is fully seatedinto the well.

Legal Information

BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

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저장 등급

10-13 - German Storage Class 10 to 13

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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프로토콜

Microscopic Examination of Samples

This protocol covers 3 modes for the microscopic examination of cell samples.

Toluidine Blue Staining Protocol for Millicell® Inserts

Toluidine blue selectively stains nuclear material and acidic tissue components, aiding in histological staining for tissues rich in DNA/RNA.

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

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SKUGTIN
PICM0RG5004053252360763